Molecular cloning and characterization of a novel retinoblastoma-binding protein
Molecular cloning and characterization of a novel retinoblastoma-binding protein
复制标题
DOI：
10.1006/geno.1998.5368
复制
发表时间：
1998-08-01
期刊：
影响因子：
4.4
通讯作者：
Zervos, AS
中科院分区：
文献类型：
--
作者：
Fusco, C;Reymond, A;Zervos, AS
我们描述了一种新的编码多肽的cDNA的分离和鉴定，这种多肽在酵母双杂交系统中以及在哺乳动物细胞中与视网膜母细胞瘤(RB)蛋白相互作用。这个新的蛋白质，我们称之为Rim，由897个氨基酸组成，有两个亮氨酸拉链基序，并且具有一个先前被确定为RE结合结构域的LECEE序列。RIM还具有一个E1A/CtBP结合基序和四个假定的核定位信号。RIM mRNA在所有受试的成人组织中普遍低水平表达，在几种肿瘤细胞系中高水平表达。Rim基因(HG；MW批准的符号RBBP8)位于人类染色体18q11.2上。(C)1998年学术出版社
We describe the isolation and characterization of a novel cDNA encoding a polypeptide that interacts in a yeast two-hybrid system as well as in mammalian cells with the retinoblastoma (RB) protein. This new protein, which we call Rim, consists of 897 amino acids, has two leucine zipper motifs, and has a LECEE sequence previously identified as an RE-binding domain. Rim also has an E1A/CtBP-binding motif and four putative nuclear localization signals. Rim mRNA is expressed ubiquitously at low levels in all human adult tissues tested and at much higher levels in several tumor cell lines. The Rim gene (HG;MW-approved symbol RBBP8) is localized on human chromosome 18q11.2. (C) 1998 Academic Press