Fungal diversity in deep-sea sediments from Magellan seamounts environment of the western Pacific revealed by high-throughput Illumina sequencing
Fungal diversity in deep-sea sediments from Magellan seamounts environment of the western Pacific revealed by high-throughput Illumina sequencing
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DOI：
10.1007/s12275-020-0198-x
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发表时间：
2020-09
影响因子：
3
通讯作者：
Shuai Yang;W. Xu;Yuanhao Gao;Xiaoyao Chen;Zhuhua Luo
中科院分区：
文献类型：
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作者：
Shuai Yang;W. Xu;Yuanhao Gao;Xiaoyao Chen;Zhuhua Luo
全球有许多海山的初级生产力远高于周边地区。然而，与其他深海环境相比，海山环境的真菌多样性探索相对较少。在本研究中，我们通过使用 ITS1 区域的高通量测序，探索了麦哲伦海山环境四个不同站的深海沉积物中的真菌群落结构。总共获得了1,897,618个ITS1序列。在这些序列中，真菌ITS1序列可以聚类成1,662个OTU。这些序列大部分属于子囊菌门。在属水平上，最丰富的属是被孢霉属（4.79%），是土壤和海洋沉积物中常见的真菌属，其次是被孢霉属。由伞形孢属(3.80%)、枝孢属(2.98%)、酵母属(2.53%)、曲霉属(2.42%)、玉蜀黍属(2.36%)、斋藤酵母属(2.20%)、木霉属(2.12%)、青霉属(2.11%)、红菇属(1.86%)、和黄萎病(1.40%)。大多数这些恢复的属属于子囊菌门。 Bray-Curtis 分析显示，每两个沉积物样本之间的真菌群落存在 37% 至 85% 的差异。主坐标分析清楚地显示了不同沉积物样品中真菌群落的变化。这些结果表明，真菌群落结构不仅在四个不同采样站之间存在差异，而且同一站的不同层之间也存在差异。深度和地理距离对真菌群落影响显着，深度和地理距离对麦哲伦海山真菌群落结构的影响基本相同。大多数真菌或多或少与植物有关，这些植物寄生/共生/内生真菌构成了不同于其他海洋生态系统的独特类型的海山环境真菌生态。
There are lots of seamounts globally whose primary production is disproportionally greater than the surrounding areas. Compared to other deep-sea environments, however, the seamounts environment is relatively less explored for fungal diversity. In the present study, we explored the fungal community structure in deep-sea sediments from four different stations of the Magellan seamounts environment by using high-throughput sequencing of the ITS1 region. A total of 1,897,618 ITS1 sequences were obtained. Among these sequences, fungal ITS1 sequences could be clustered into 1,662 OTUs. The majority of these sequences belonged to Ascomycota. In the genera level, the most abundant genus wasMortierella(4.79%), which was reported as a common fungal genus in soil and marine sediments, followed byUmbelopsis(3.80%),Cladosporium(2.98%),Saccharomycopsis(2.53%),Aspergillus(2.42%),Hortaea(2.36%),Saitozyma(2.20%),Trichoderma(2.12%),Penicillium(2.11%),Russula(1.86%), andVerticillium(1.40%). Most of these recovered genera belong to Ascomycota. The Bray-Curtis analysis showed that there was 37 to 85% dissimilarity of fungal communities between each two sediment samples. The Principal coordinates analysis clearly showed variations in the fungal community among different sediment samples. These results suggested that there was a difference in fungal community structures not only among four different sampling stations but also for different layers at the same station. The depth and geographical distance significantly affect the fungal community, and the effect of depth and geographical distance on the structure of the fungal community in the Magellan seamounts is basically same. Most of the fungi were more or less related to plants, these plant parasitic/symbiotic/endophytic fungi constitute a unique type of seamounts environmental fungal ecology, different from other marine ecosystems.