Effects of IL-15 on rat brown adipose tissue:: Uncoupling proteins and PPARs
Effects of IL-15 on rat brown adipose tissue:: Uncoupling proteins and PPARs
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DOI：
10.1038/oby.2007.47
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发表时间：
2008-02-01
期刊：
影响因子：
6.9
通讯作者：
Lopez-Soriano, Francisco J.
中科院分区：
文献类型：
--
作者：
Almendro, Vanessa;Fuster, Gemma;Lopez-Soriano, Francisco J.
目的：白细胞介素 15 (IL-15) 在脂质代谢中发挥重要作用，因为给大鼠施用会导致白色脂肪组织 (WAT) 显着减少。这种脂肪量的减少似乎是由低甘油三酯血症引起的，并且与低甘油三酯血症相关，这是由于肝脏脂肪生成减少和脂肪酸氧化增加造成的。我们之前观察到 IL-15 治疗会诱导肌肉中解偶联蛋白 (UCP) 的表达。本研究的目的是研究 IL-15 对棕色脂肪组织 (BAT) 的影响，特别是对该组织中脂质代谢相关基因的影响。方法和程序：雄性 Wistar 大鼠每天接受 IL-15 治疗，持续 7 天。收集脂肪组织，并在 BAT 上评估 UCP、过氧化物酶体增殖物激活受体 (PPAR) 和几个与脂肪酸转运和氧化有关的基因的 mRNA 含量。 结果：IL-15 治疗大鼠导致 WAT 和 BAT 质量减少（分别为 35% 和 24%）。在 BAT 中，生热蛋白（UCP1 和 UCP3）、脂质相关转录因子（PPAR δ 和 PPAR α）以及其他参与膜转运的蛋白（脂肪酸转位酶 (FAT) 和脂肪酸转运蛋白 (FATP)）、线粒体转运（肉毒碱棕榈酰转移酶-I (CPT-I) 和 作为治疗的结果，观察到了 CPT-II）和脂肪酸的消耗（酰基辅酶 A 合成酶 4 (ACS4)）。讨论：在 BAT 中观察到的变化表明，IL-15 可能通过调节脂质氧化和可能的生热作用（由 UCP 和 PPAR 介导的过程）参与该组织中的脂质消耗。
Objectives: Interleukin-15 (IL-15) plays an important role in lipid metabolism as its administration to rats causes a marked depletion of white adipose tissue (WAT). This reduction in fat mass seems to be caused by and related to hipotriglyceridemia as a result of a lower hepatic lipogenesis and an increased fatty acid oxidation. We have previously observed that IL-15 treatment induces the expression of uncoupling proteins (UCPs) in muscle. The aim of this study was to investigate the effects of IL-15 on brown adipose tissue (BAT), and in particular on genes related to lipid metabolism in this tissue.Methods and Procedures: Male Wistar rats were treated daily with IL-15 for 7 days. Adipose tissues were collected and the mRNA content of UCPs, peroxisome proliferator-activated receptors (PPARs) and several genes implicated in fatty acid transport and oxidation were evaluated on BAT.Results: IL-15 treatment in rats causes a decrease in the mass of both WAT and BAT (35 and 24%, respectively). In BAT, an important upregulation of the mRNA content of thermogenic proteins (UCP1 and UCP3), lipid-related transcription factors (PPAR delta and PPAR alpha) and other proteins implicated in membrane transport (fatty acid translocase (FAT) and fatty acid transport protein (FATP)), mitochondrial transport (carnitine palmitoyl transferase-I (CPT-I) and CPT-II) and consumption (acyl-CoA synthetase 4 (ACS4)) of fatty acids was observed as a consequence of the treatment.Discussion: The changes observed in BAT suggest that IL-15 could be implicated in lipid consumption in this tissue by regulating lipid oxidation and probably thermogenesis, processes mediated by UCPs and PPARs.