DIGESTION AND ABSORPTION OF SUGARS AND SUGAR SUBSTITUTES IN RAT SMALL-INTESTINE
DIGESTION AND ABSORPTION OF SUGARS AND SUGAR SUBSTITUTES IN RAT SMALL-INTESTINE
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DOI：
10.3177/jnsv.32.93
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发表时间：
1986-02-01
影响因子：
1.6
通讯作者：
MORIUCHI, S
中科院分区：
文献类型：
--
作者：
TSUJI, Y;YAMADA, K;MORIUCHI, S
新开发的糖替代品的生物利用度是通过测量钠离子依赖的葡萄糖主动转运引起的跨壁电位差（delta . pd）来观察的，这被认为是糖替代品水解产生的。采用成年大鼠空肠制备的肠膨出囊测定PD，并与肠膨出囊粘膜匀浆中糖代用品的消化率进行比较。葡萄糖、麦芽糖或麦芽糖果糖诱发的pd水平几乎相同。蔗糖引起的PD值稍低。麦芽糖醇和巴丁糖诱发的pd值较低，δ δ。低聚果糖引起的PDs可以忽略不计。粘膜匀浆对这些糖和糖替代品的水解活性与δ δ - pd相关。. delta与。各种糖和糖替代品的PDmax及其相应水解活性的Vmax。此外，这些糖的Kt值和Km值之间也存在显著的正相关。这些结果表明，糖替代品的吸收依赖于膜消化酶的消化率。
The bioavailabiilty of newly developed sugar substitutes was observed by measuring the transmural potential difference (.DELTA.PD) evoked by Na+-dependent active transport of glucose, which is supposed to be produced by the hydrolysis of sugar substitutes. .DELTA.PD was measured using everted intestinal sac prepared from jejunum of adult rats and compared with the digestibility of sugar substitutes in the mucosal homogenate of everted sac. .DELTA.PDs evoked by glucose, maltose or maltosylfructose had almost the same levels, however, the .DELTA.PD evoked by sucrose was a little lower. .DELTA.PDs evoked by maltitol or palatinose were low, and .DELTA.PDs evoked by fructooligosaccharides were negligible. The hydrolyzing activities of these sugars and sugar substitutes by the mucosal homogenate were correlated with the .DELTA.PDs. A significant positive correlation was observed between .DELTA.PDmax of various sugars and sugar substitutes and the Vmax of their corresponding hydrolyzing activities. Also, a significant positive correlation was observed between Kt and Km values of these sugars. These results suggest that the absorption of sugar substitutes is dependent on digestibility by membrane digestive enzymes.