Misidentification rate in the Israeli dairy cattle population and its implications for genetic improvement
Misidentification rate in the Israeli dairy cattle population and its implications for genetic improvement
复制标题
DOI：
10.3168/jds.s0022-0302(96)76413-5
复制
发表时间：
1996-04-01
影响因子：
3.5
通讯作者：
Weller, JI
中科院分区：
文献类型：
--
作者：
Ron, M;Blanc, Y;Weller, JI
DNA微卫星标记可有效地用于不需要对母畜进行基因分型的牛的亲子鉴定。利用102头AI公畜的母系等位基因，测定了12个微卫星位点的群体等位基因频率。最常见的微卫星等位基因的频率范围为0.27至0.58。大多数基因座至少有一个等位基因，只存在于一个单一的个人。排除了9/173头奶牛（5.2%）和3/102头船体（2.9%）的父系，因为后代中至少有一个基因座不存在推定的父系等位基因。对于9头奶牛中的4头和所有3头公牛，排除是基于至少两个基因座。平均排除概率为0.85奶牛和0.99公牛。假设每个基因型的成本为5美元，错误识别率为5%，折扣率为0.05，以色列-荷斯坦育种计划从每个年轻公鹿的100个测试女儿的基因分型中获得的额外利润在10年内变为阳性，20年后达到近240万美元。
The DNA microsatellites can be efficiently used to determine incorrect paternity attribution of cattle without genotyping of dams. Allelic frequencies of the population were determined for 12 microsatellites using the maternal alleles of 102 AI sires. The frequency of the most common microsatellite allele ranged from 0.27 to 0.58. Most loci had at least one allele that was present in only a single individual. Paternity of 9 of 173 cows (5.2%) and 3 of 102 hulls (2.9%) was excluded because putative paternal alleles were not present in progeny for at least one locus. For 4 of the 9 cows and all 3 bulls, exclusion was based on at least two loci. Mean probability of exclusion was 0.85 for cows and 0.99 for bulls. With an assumed cost of US $5 per genotype, a misidentification rate of 5%, and a discount rate of 0.05, additional profit for the Israeli-Holstein breeding program from genotyping 100 test daughters of each young sire becomes positive within 10 yr and reaches nearly US $2.4 million after 20 yr.