Biocontrol potential of Agromyces allii 130935 and its metabolites against root-knot nematode Meloidogyne incognita
Biocontrol potential of Agromyces allii 130935 and its metabolites against root-knot nematode Meloidogyne incognita
复制标题
蒜苗130935及其代谢物对根结线虫根结线虫的生防潜力
DOI：
10.1016/j.rhisph.2021.100378
复制
发表时间：
2021-05-14
期刊：
影响因子：
3.7
通讯作者：
Liu, Yajun
中科院分区：
文献类型：
--
作者：
Cao, Xiaomei;Zhang, Ruiping;Liu, Yajun
南方根结线虫是一种对农作物造成严重危害的根结线虫。从地衣中分离到一株放线菌Agromyces allii 130935，该放线菌具有杀线虫活性。无名氏A.乙酸乙酯相的杀线虫活性大蒜130935在8h和24 h分别达到89%和96%。从A的乙酸乙酯相中分离鉴定了4个化合物，分别为2-羟基十一酸、4-（2-呋喃羰基）-3-羟基-5-苯基-2（5 H）-呋喃酮、1-（1H-吡咯-2-基）-甲烷和2-[（5-硝基-2-呋喃基）羰基]氨基]氧基]-丙酸。allii 130935. 4种化合物对中华绒螯蟹二龄幼虫均有一定的毒性作用。24 h时不明。化合物2-[[[（5-硝基-2-呋喃基）羰基]氨基]氧基]-丙酸在200 μ g mL（-1）浓度下在24小时内引起50%的死亡率。田间试验证实，A.大蒜130935显著降低根瘿，防效为50%。结果表明，A.大蒜130935及其代谢产物是潜在的生防微生物。无名氏
Meloidogyne incognita is a root-knot nematode that causes serious damage to crops. An actinomycete, Agromyces allii 130935, isolated from lichens exhibited nematicidal activity against second-stage juveniles of M. incognita. The nematicidal activity of the ethyl acetate phase of A. allii 130935 reached 89% and 96% at 8 h and 24 h, respectively. Four compounds, namely 2-hydroxyundecanoic acid, 4-(2-furanylcarbonyl)-3-hydroxy-5-phenyl-2 (5H)-furanone, 1-(1H-pyrrol-2-yl)-methanediol, and 2-[[[(5-nitro-2-furanyl)carbonyl]amino]oxy]-propanoic acid, were isolated and identified from the ethyl acetate phase of A. allii 130935. All the four compounds showed a certain toxic effect against the second-stage juveniles of M. incognita at 24 h. Compound 2-[[[(5-nitro-2-furanyl)carbonyl]amino]oxy]-propanoic acid caused 50% mortality at a concentration of 200 mu g mL(-1) within 24 h. Field experiments confirmed that the ethyl acetate extract of A. allii 130935 significantly reduced root galls, with a control effect 50%. Our results indicate that A. allii 130935 and its metabolites are potential biocontrol agents against M. incognita.