Effects of estrogens on MCF-7 cells: positive or negative regulation by the nature of the ligand-receptor complex.
Effects of estrogens on MCF-7 cells: positive or negative regulation by the nature of the ligand-receptor complex.
复制标题
雌激素对 MCF-7 细胞的影响：配体-受体复合物性质的正向或负向调节。
DOI：
10.1016/0006-291x(91)90516-a
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发表时间：
1991
影响因子：
3.1
通讯作者：
Fishman,J
中科院分区：
文献类型：
--
作者：
Swaneck,GE;Fishman,J
本研究表明，雌激素的雌激素受体（ER）的结合域的结合的性质修改的雌激素依赖性细胞的反应。我们在这里报告，10 nM雌二醇（E2）与ER形成非共价结合，在短期或长期暴露于E2后，增加培养的ER+MCF-7人乳腺癌细胞中ER和PR的水平。相比之下，10 nM 16-α-羟基雌酮（16α-OHE 1），E2的生理代谢产物，在短期培养中与E2相当，但在长期孵育后，16α-OHE 1与ER形成共价结合，使ER和PR水平显著降低，达到与对照细胞相似或低于对照细胞的水平。
The present study demonstrates that the nature of the binding of estrogens to the hormone-binding domain of the estrogen receptor (ER) modifies the responses of estrogen-dependent cells. We report here that 10 nM estradiol (E2) forms noncovalent associations with the ER, increases the level of ER and Progesterone Receptors (PR) in ER+MCF-7 human breast cancer cells in culture following short-term or long-term exposure to E2. In contrast, 10 nM 16-alpha-hydroxyestrone (16α-OHE1), a physiological metabolite of E2, in short-term cultures is equivalent to E2, but upon long-term incubation, 16α-OHE1forms covalent associations with the ER, produces a marked decrease in ER and PR levels reaching values similar to, or below to that of control cells.