Cooperative adsorption of proteins onto hydroxyapatite
Cooperative adsorption of proteins onto hydroxyapatite
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DOI：
10.1006/jcis.1997.5364
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发表时间：
1998-04-01
影响因子：
9.9
通讯作者：
Andrade, JD
中科院分区：
文献类型：
--
作者：
Luo, QL;Andrade, JD
蛋白质吸附等温线通常由朗缪尔方程描述。蛋白质吸附通常是一个协同过程。虽然简单的朗缪尔方程通常不包括协同性，但希尔图可以表示协同性，包括协同性的程度。我们对溶菌酶、白蛋白、转铁蛋白和乳铁蛋白在羟基磷灰石上吸附的研究表明，协同性可以描述为正（n > 1.5）、部分正（1 < n < 1.5）、部分负（1 < n < 0.5）和负（n < 0.5）。随着蛋白质浓度的增加，有三个阶段（i，ii和iii），类似于在氧和血红蛋白之间的相互作用中观察到的协同性。阶段的数量由蛋白质的结构刚性和蛋白质之间以及蛋白质与吸附剂之间的横向相互作用决定。我们的希尔图显示，在大多数情况下，阶段ii具有最低的协同度，阶段iii具有最高的协同度，阶段i介于两者之间。在阶段i，当蛋白质溶液浓度较低时，低协同度与吸附等温线中的线性范围高度一致。这些结果为分析吸附色谱的设计和优化提供了有用的信息。确定协同度的因素包括蛋白质构象，pI，和溶液pH值。我们现在定量地了解这些蛋白质对羟基磷灰石的协同吸附，协同度，以及如何控制吸附过程。(C)北京：科学出版社.
Protein adsorption isotherms are often described by the Langmuir equation. Protein adsorption is generally a cooperative process. Although the simple Langmuir equation does not normally include cooperativity, the Hill plot can represent cooperativity, including the degree of cooperativity. Our studies of the adsorption of lysozyme, albumin, transferrin, and lactoferrin onto hydroxyapatite suggest that cooperativity can be described as positive (n > 1.5), partially positive (1 < n < 1.5), partially negative (1 < n < 0.5), and negative (n < 0.5). There are three stages (i, ii, and iii) as protein concentration increases, similar to the cooperativity observed in the interaction between oxygen and hemoglobin. The number of stages is determined by the structural rigidity of proteins and by the lateral interactions between proteins and between protein and adsorbent. Our Hill plots show that stage ii has the lowest degree of cooperativity, stage iii has the highest, and stage i is in between, for most cases. In stage i the low degree of cooperativity is highly consistent with the linear range in the adsorption isotherm when protein solution concentration is low. These results provide useful information for the design and optimization of analytical adsorption chromatography. The factors determining the degree of cooperativity include protein conformation, pI, and solution pH. We now quantitatively understand the cooperative adsorption of these proteins on hydroxyapatite, the degree of cooperativity, and how to control the adsorption process. (C) 1998 Academic Press.