Coordinated regulation of infection-related morphogenesis by the KMT2-Cre1-Hyd4 regulatory pathway to facilitate fungal infection
Coordinated regulation of infection-related morphogenesis by the KMT2-Cre1-Hyd4 regulatory pathway to facilitate fungal infection
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KMT2-Cre1-Hyd4 调节途径协调调节感染相关形态发生以促进真菌感染
DOI：
10.1126/sciadv.aaz1659
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发表时间：
2020-03-01
期刊：
影响因子：
13.6
通讯作者：
Wang, Sibao
中科院分区：
文献类型：
--
作者：
Lai, Yiling;Cao, Xuan;Wang, Sibao
KMT2-Cre1-Hyd4调控途径调控杀虫真菌感染相关的形态发生和致病性。昆虫病原真菌可以克服杀虫剂抗性，是控制蚊子的有前途的工具。更好地了解真菌与蚊子的相互作用对提高真菌的功效至关重要。在昆虫角质层诱导下，病原真菌发生了明显的与感染相关的形态分化。然而，真菌感染相关形态发生的调控机制尚不清楚。在这里，我们发现罗伯特绿僵菌中的一种组蛋白赖氨酸甲基转移酶KMT2 （MrKMT2）在角质层诱导后上调。MrKMT2在蚊子表皮感染过程中通过H3K4三甲基化上调转录因子Mrcre1基因，在调节感染相关的形态发生和致病性中发挥重要作用。MrCre1进一步调控角质层诱导基因Mrhyd4，调节感染结构（附着胞）的形成和毒力。总体而言，MrKMT2-MrCre1-MrHyd4调控途径调控罗伯特氏杆菌感染相关的形态发生和致病性。这些发现揭示了表观遗传调控机制在昆虫真菌发病机制中起着关键作用，并为病原真菌与昆虫宿主的分子相互作用提供了新的认识。
The KMT2-Cre1-Hyd4 regulatory pathway modulates infection-related morphogenesis and pathogenicity in an insecticidal fungus. Entomopathogenic fungi can overcome insecticide resistance and represent promising tools for the control of mosquitoes. Better understanding of fungus-mosquito interactions is critical for improvement of fungal efficacy. Upon insect cuticle induction, pathogenic fungi undergo marked infection-related morphological differentiation. However, regulatory mechanisms of fungal infection–related morphogenesis are poorly understood. Here, we show that a histone lysine methyltransferase KMT2 in Metarhizium robertsii (MrKMT2) is up-regulated upon cuticle induction. MrKMT2 plays crucial roles in regulating infection-related morphogenesis and pathogenicity by up-regulating the transcription factor gene Mrcre1 via H3K4 trimethylation during mosquito cuticle infection. MrCre1 further regulates the cuticle-induced gene Mrhyd4 to modulate infection structure (appressorium) formation and virulence. Overall, the MrKMT2-MrCre1-MrHyd4 regulatory pathway regulates infection-related morphogenesis and pathogenicity in M. robertsii. These findings reveal that the epigenetic regulatory mechanism plays a pivotal role in regulating fungal pathogenesis in insects, and provide new insights into molecular interactions between pathogenic fungi and insect hosts.