Tongue Muscle-Derived Stem Cells Express Connexin 43 and Improve Cardiac Remodeling and Survival After Myocardial Infarction in Mice
Tongue Muscle-Derived Stem Cells Express Connexin 43 and Improve Cardiac Remodeling and Survival After Myocardial Infarction in Mice
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DOI：
10.1253/circj.cj-10-0033
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Matsuzaki, Masunori
中科院分区：
文献类型：
--
作者：
Shibuya, Masaki;Miura, Toshiro;Matsuzaki, Masunori
背景资料：心力衰竭的细胞移植治疗受到心肌细胞分化不良和连接蛋白43（Cx43）表达不良引起的心律失常的阻碍。方法和结果：从正常和绿色荧光蛋白（GFP）转基因小鼠舌中分离舌肌来源的Sca-1（+）细胞（TDSCs），使用表面抗原Sea-1。通过测量钙瞬变和心脏特异性基因的表达来证实心肌分化。缝隙连接的形成通过Cx43的表达和染料转移法证实。再生细胞的收缩通过钙瞬变来证明。将GFP小鼠来源的TDSC移植到急性心肌梗死模型的心脏中。移植后3个月，与对照组相比，移植组左室重构明显减轻，存活率明显提高。结论：TDSC可形成缝隙连接，改善心肌梗死后心功能和长期存活。(Circ J 2010; 74：1219-1226）
Background: Cell transplantation therapy for heart failure is hindered by poor differentiation into cardiomyocytes and arrhythmias caused by the poor expression of connexin 43 (Cx43). A new stem cell source for cardiac regeneration is needed.Methods and Results: Tongue muscle-derived Sca-1(+) cells (TDSCs) were isolated from normal and green fluorescence protein (GFP)-transgenic mouse tongues using surface antigen Sea-1. Cardiomyogenic differentiation was confirmed by measuring the calcium transient and the expression of cardiac-specific genes. The formation of gap junctions was confirmed by the expression of Cx43 and the dye transfer method. The contraction of regenerated cells was demonstrated by the calcium transients. GFP mouse-derived TDSCs were transplanted into hearts in a model of acute myocardial infarction. Three months after transplantation, LV remodeling was attenuated and the survival rate was improved compared with the control group.Conclusions: TDSCs form gap junctions and improve cardiac function and long-term survival after myocardial infarction. (Circ J 2010; 74: 1219-1226)