In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes
In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes
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DOI：
10.1007/s10482-008-9263-x
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Vagvolgyi, Csaba
中科院分区：
文献类型：
--
作者：
Galgoczy, Laszlo;Papp, Tamas;Vagvolgyi, Csaba
选择犬小孢子菌、石膏样小孢子菌、须癣毛癣菌、红色毛癣菌和断端毛癣菌5种皮肤癣菌菌株，进行产黄青霉菌抗真菌蛋白（PAF）及其与氟康唑（FCZ）的联合抗菌试验。当PAF单独使用或以恒定浓度（100 μ g ml（-1））与FCZ（0.25 ~ 32 μ g ml（-1））组合使用时，检测到PAF的MIC为1.56 ~ 200 μ g ml（-1），对小分生孢子萌发和生长的抑制。FCZ的MIC在0.25 ~ 128 μ g/ml之间。PAF在200 μ g ml（-1）时产生杀真菌作用，在50至200 μ g ml（-1）时抑制生长。在64 μ g ml（-1）的FCZ下检测到M的总生长抑制和真菌抑制活性。gypseum，T. mentagrophytes和T.在32 μ g ml（-1）FCZ下，M. canis和T.红色。PAF和FCZ对除M. gypseum，其中未检测到相互作用。
Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 mu g ml(-1) when it was used alone, or at constant concentration (100 mu g ml(-1)) in combination with FCZ at from 0.25 to 32 mu g ml(-1). The MICs for FCZ were found to be between 0.25 and 128 mu g ml(-1). PAF caused a fungicidal effect at 200 mu g ml(-1) and reduced growth at between 50 and 200 mu g ml(-1). Total growth inhibition with fungistatic activity was detected at 64 mu g ml(-1) of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 mu g ml(-1) FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.