Sequence of the 3′-terminal end (8•1 kb) of the genome of porcine haemagglutinating encephalomyelitis virus:: comparison with other haemagglutinating coronaviruses
Sequence of the 3′-terminal end (8•1 kb) of the genome of porcine haemagglutinating encephalomyelitis virus:: comparison with other haemagglutinating coronaviruses
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DOI：
10.1099/0022-1317-83-10-2411
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发表时间：
2002-10-01
影响因子：
3.8
通讯作者：
Dea, S
中科院分区：
文献类型：
--
作者：
Sasseville, AMJ;Boutin, M;Dea, S
一种致细胞病变的冠状病毒，经血清学鉴定为猪血凝性脑脊髓炎病毒（HEV），最近与加拿大魁北克南部和安大略养猪场哺乳小猪的消瘦和脑炎急性爆发有关。对在HRT-18细胞中繁殖的原型HEV-67 N株和最近的魁北克IAF-404田间分离株的基因组的3 '末端进行测序。总体而言，测序数据表明，自1962年在北美首次分离以来，HEV在抗原性和遗传学上保持稳定。与牛肠道致病性冠状病毒（BCoV）的原型株相比，HEV和人呼吸道冠状病毒（HCoV-OC 43）的ORF 4基因均发生了较大的缺失。推导的氨基酸序列与BCoV和HCoV-OC 43的结构蛋白HE、S、M和N的同源性分别为89/88、80、93/92和95/94%。两种戊型肝炎病毒株的S基因的S1部分中观察到主要变异，与其他两种血凝性冠状病毒相比，只有73/71%的氨基酸同一性。
A cytopathogenic coronavirus, serologically identified as porcine haemagglutinating encephalomyelitis virus (HEV), has recently been associated with acute outbreaks of wasting and encephalitis in nursing piglets from pig farms in southern Quebec and Ontario, Canada. The 3'-terminal end of the genome of the prototype HEV-67N strain and that of the recent Quebec IAF-404 field isolate, both propagated in HRT-18 cells, were sequenced. Overall, sequencing data indicated that HEV has remained antigenically and genetically stable since its first isolation in North America in 1962. Compared with the prototype strain of bovine enteropathogenic coronavirus (BCoV), HEV, as well as the human respiratory coronavirus (HCoV-OC43) showed a major deletion in their ORF4 gene. Deduced amino acid sequences for both HEV strains revealed 89/88, 80, 93/92 and 95/94% identities with the structural proteins HE, S, M and N of BCoV and HCoV-OC43, respectively. Major variations were observed in the S1 portion of the S gene of both HEV strains, with only 73/71% amino acid identities compared with those of the two other haemagglutinating coronaviruses.