Actin-myosin network is required for proper assembly of influenza virus particles
Actin-myosin network is required for proper assembly of influenza virus particles
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DOI：
10.1016/j.virol.2014.12.016
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发表时间：
2015-02-01
期刊：
影响因子：
3.7
通讯作者：
Nagata, Kyosuke
中科院分区：
文献类型：
--
作者：
Kumakura, Michiko;Kawaguchi, Atsushi;Nagata, Kyosuke
已知肌动蛋白丝在细胞动力学中起核心作用。肌动蛋白聚合后，各种肌动蛋白交联蛋白，包括非肌肉肌球蛋白II促进空间组织的肌动蛋白丝网络的形成。肌动蛋白-肌球蛋白网络在质膜下高度扩张。流感病毒的基因组（vRNA）在细胞核中复制。然后，新合成的vRNA作为核糖核蛋白复合物（vRNP）被核输出到细胞质，然后运输到质膜下，在那里病毒颗粒组装。在这里，我们发现，通过抑制肌动蛋白-肌球蛋白网络的形成，病毒滴度趋于降低，HA病毒刺突蛋白聚集在质膜上。这些结果表明肌动蛋白-肌球蛋白网络在病毒形成中起重要作用。(C)2014 Elsevier Inc. All rights reserved.
Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation. (C) 2014 Elsevier Inc. All rights reserved.