Characterization of bacterial adhesin interactions with extracellular matrix components utilizing biosensor technology
Characterization of bacterial adhesin interactions with extracellular matrix components utilizing biosensor technology
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DOI：
10.1006/meth.1994.1016
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Hook, Magnus
中科院分区：
文献类型：
--
作者：
House-Pompeo, Karen;Boles, Jeffey Oakley;Hook, Magnus
微生物对宿主组织的粘附是感染过程的第一步，是开发新的抗菌药物的潜在目标。我们的实验室专注于微生物粘附宿主细胞外基质成分的分子机制，这些成分涉及特定的细菌表面蛋白，粘附素与宿主ECM分子上的不同位点相互作用。BIAcore是我们用来描述这些相互作用的技术之一。在本文中，我们描述了使用BIAcore来(a)确定不同形式的重组胶原粘连蛋白的结合常数和特异性，这些重组胶原粘连蛋白都含有活性位点，但侧翼序列的数量不同；(b)比较来自不同细菌的四种纤维连接蛋白粘连蛋白的重组活性位点的配体结合动力学。
Microbial adhesion to host tissue represents the first step in an infection process and is a potential target for the development of new antimicrobial agents. Our laboratory has focused on the molecular mechanisms of microbial adhesion to host extracellular matrix components that involve specific bacterial surface proteins, adhesins that interact with distinct sites on host ECM molecules. BIAcore is one of the techniques we use to characterize these interactions. In this communication we describe the use of BIAcore to (a) determine the binding constants and specificity of different forms of a recombinant collagen adhesin that all contain the active site but vary in amount of flanking sequence and (b) compare the ligand-binding kinetics of recombinant active sites of four fibronectin-binding adhesins from different bacteria.