Autocrine role of insulin-like growth factor (IGF)-I in a human thyroid cancer cell line.
Autocrine role of insulin-like growth factor (IGF)-I in a human thyroid cancer cell line.
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DOI：
10.1016/0959-8049(92)90033-x
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发表时间：
1992
影响因子：
8.4
通讯作者：
N. Onoda;E. Ohmura;T. Tsushima;Y. Ohba;N. Emoto;O. Isozaki;Ya. Sato;K. Shizume;H. Demura
中科院分区：
文献类型：
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作者：
N. Onoda;E. Ohmura;T. Tsushima;Y. Ohba;N. Emoto;O. Isozaki;Ya. Sato;K. Shizume;H. Demura
研究了来源于人甲状腺乳头状癌细胞的已建立的细胞系（TC-细胞，克隆78）的肽生长因子生产。这些细胞具有胰岛素样生长因子-I（IGF-I）的特异性结合位点，并对这种生长因子产生增殖反应。发现TC细胞条件培养基含有胰岛素样生长因子（IGF）-I和IGF结合蛋白。逆转录-聚合酶链反应显示IGF-I mRNA的表达。当加入IGF-1受体单克隆抗体（α IR 3）时，TC细胞在无血清培养液中的生长明显减少。当从培养基中除去抗体时，细胞的生长速率恢复。这些结果强烈表明TC细胞产生IGF-I，IGF-I参与调节其自身的生长。
An established cell line (TC-cell, clone 78) derived from human thyroid papillary cancer cells was investigated for production of peptide growth factors. The cells had specific binding sites for insulin-like growth factor-I (IGF-I) and responded to this growth factor with increased proliferation. Culture medium conditioned by TC cells was found to contain insulin-like growth factor (IGF)-I and IGF-binding protein(s). Furthermore, reverse transcription-polymerase chain reaction revealed expression of IGF-I mRNA. When monoclonal antibody to IGF-I receptors (αIR3) was added, the growth of TC cells cultured in serum-free medium was significantly reduced. The growth rate of the cells was restored when the antibody was removed from the medium. These results strongly suggest that TC cells produce IGF-I, which is involved in the regulation of their own growth.