Ultraviolet A-specific induction of anthocyanin biosynthesis in the swollen hypocotyls of turnip (Brassica rapa).
Ultraviolet A-specific induction of anthocyanin biosynthesis in the swollen hypocotyls of turnip (Brassica rapa).
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DOI：
10.1093/jxb/erm036
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发表时间：
2007-05
影响因子：
6.9
通讯作者：
Bo Zhou;Yuhua Li;Zhiru Xu;Haifang Yan;S. Homma;S. Kawabata
中科院分区：
文献类型：
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作者：
Bo Zhou;Yuhua Li;Zhiru Xu;Haifang Yan;S. Homma;S. Kawabata
研究了紫外线A（UV-A）对红萝卜津田（Tsuda）膨大下胚轴花青苷生物合成的调控。将含有可忽略的花青素苷的遮蔽的肿胀下胚轴暴露于包括低通量UV-B、UV-A、蓝色、红色、远红色、红色加UV-A、远红色加UV-A和蓝色加红色的人工光源。其中，只有UV-A能诱导花青苷的合成，而红光或远红光与UV-A共辐射对UV-A诱导花青苷合成的影响不大。苯丙氨酸解氨酶（PAL; EC 4.3.1.5）、查耳酮合酶（CHS; EC 2.3.1.74）、黄烷酮3-羟化酶（F3 H; EC 1.14.11.9）、二氢黄酮醇4-还原酶（DFR; EC 1.1.1.219）和花青素合酶（ANS; EC 1.14.11.19）基因的表达在24 h暴露于UV-A期间随时间增加。相反，用红色、蓝色、UV-B以及蓝色与红色的组合照射未能诱导CHS表达。微阵列分析表明，只有少数基因，包括CHS和F3 H，显着诱导UV-A，而一组独立的许多基因诱导低通量UV-B。与蓝光和UV-B相比，UV-A特异性诱导的花青素苷生物合成和独特的基因表达谱表明，观察到的诱导花青素苷生物合成的红萝卜是由一个独特的UV-A特异性光感受器介导的，而不是由光敏色素，UV-A/蓝光光感受器，或UV-B光感受器。
Ultraviolet A (UV-A)-mediated regulation of anthocyanin biosynthesis was investigated in swollen hypocotyls of the red turnip 'Tsuda'. The shaded swollen hypocotyls which contained negligible anthocyanin were exposed to artificial light sources including low fluence UV-B, UV-A, blue, red, far-red, red plus UV-A, far-red plus UV-A, and blue plus red. Among these lights, only UV-A induced anthocyanin biosynthesis and co-irradiation of red or far-red with UV-A did not affect the extent of UV-A-induced anthocyanin accumulation. The expression of phenylalanine ammonia lyase (PAL; EC 4.3.1.5), chalcone synthase (CHS; EC 2.3.1.74), flavanone 3-hydroxylase (F3H; EC 1.14.11.9), dihydroflavonol 4-reductase (DFR; EC 1.1.1.219), and anthocyanidin synthase (ANS; EC 1.14.11.19) genes was increased with time during a 24 h exposure to UV-A. In contrast, irradiation with red, blue, UV-B, and a combination of blue with red failed to induce CHS expression. Microarray analysis showed that only a few genes, including CHS and F3H, were induced significantly by UV-A, while a separate set of many genes was induced by low fluence UV-B. The UV-A-specific induction of anthocyanin biosynthesis and the unique gene expression profile upon UV-A irradiation as compared with blue and UV-B demonstrated that the observed induction of anthocyanin biosynthesis in red turnips was mediated by a distinct UV-A-specific photoreceptor, but not by phytochromes, UV-A/blue photoreceptors, or UV-B photoreceptors.