TRANSCRIPTION OF A SATELLITE DNA IN THE NEWT
TRANSCRIPTION OF A SATELLITE DNA IN THE NEWT
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DOI：
10.1083/jcb.103.4.1137
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发表时间：
1986-10-01
影响因子：
7.8
通讯作者：
GALL, JG
中科院分区：
文献类型：
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作者：
EPSTEIN, LM;MAHON, KA;GALL, JG
Statllite 2是Notophthalmus viri下降蝾螈基因组中一个丰富的、330 bp的连续重复序列。这个序列分布在整个基因组的11条染色体上。在卵形成过程中，两条卫星2链都在灯刷染色体上转录，这可能是上游结构基因启动子读取的结果。除了这些异质核转录本外，卫星2在多种不同组织中与稳定的、链特异性的细胞质转录本同源。这些转录本中的大多数在大小上与整个卫星2重复单元相对应，或者与重复的整个倍数相对应。通过引物延伸对卵巢中存在的转录本进行了测序，发现其同源性比8个独立克隆的卫星2 DNA重复序列更强。我们认为稳定的细胞质转录本是由一小部分基因组卫星2序列编码的。
Statllite 2 is an abundant, 330-bp tandemly repeated sequence in the genome of the newt, Notophthalmus viridescens. This sequence is distributed throughout the genome on each of the 11 chromosomes. Both strands of satellite 2 are transcribed on the lampbrush chromosomes during oogenesis, probably as a result of readthrough from upstream structural gene promoters. In addition to these heterogeneous nuclear transcripts, satellite 2 is homologous to stable, strand-specific cytoplasmic transcripts in a variety of different tissues. The majority of these transcripts correspond in size to the entire satellite 2 repeat unit, or to whole multiples of the repeat. The transcripts present in the ovary have been sequenced by primer extension and were found to be more homogeneous than eight independently cloned satellite 2 DNA repeats. We propose that the stable cytoplasmci transcripts are encoded by a small subset of genomic satellite 2 sequences.