Synthesis of oligodeoxynucleotides containing a single diastereoisomer of alpha-(N2-2'-deoxyguanosinyl)-N-desmethyltamoxifen.
Synthesis of oligodeoxynucleotides containing a single diastereoisomer of alpha-(N2-2'-deoxyguanosinyl)-N-desmethyltamoxifen.
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含有α-(N2-2-脱氧鸟苷基)-N-去甲基他莫昔芬单一非对映异构体的寡脱氧核苷酸的合成。
DOI：
10.1016/j.bmcl.2004.05.030
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Shibutani,Shinya
中科院分区：
文献类型：
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作者：
Laxmi,YRSantosh;Suzuki,Naomi;Kim,SungYeon;Shibutani,Shinya
描述了一种含有 (E)-α-(N2-脱氧鸟苷基)-N-去甲基他莫昔芬非对映异构体的寡脱氧核苷酸的亚磷酰胺化学合成方法，这是一种主要的他莫昔芬 (TAM) 衍生的 DNA 加合物，适用于接受 TAM 治疗的动物和妇女。位点特异性修饰的寡脱氧核苷酸可用于诱变、DNA 修复和 3D 结构研究，也可作为动物和人类 TAM-DNA 加合物定量分析的标准。
A phosphoramidite chemical synthesis of oligodeoxynucleotides containing a diastereoisomer of (E)-α-(N2-deoxyguanosinyl)-N-desmethyltamoxifen, a major tamoxifen (TAM)-derived DNA adduct in animal and women treated with TAM, was described. The site-specifically modified oligodeoxynucleotide can be used for mutagenesis, DNA repair, and 3D structural studies and also as standard for quantitative analysis of TAM-DNA adducts in animal and human.