Target Delivery and Cell Imaging Using Hyaluronic Acid-Functionalized Graphene Quantum Dots
Target Delivery and Cell Imaging Using Hyaluronic Acid-Functionalized Graphene Quantum Dots
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DOI：
10.1021/mp400219u
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发表时间：
2013-10-01
影响因子：
4.9
通讯作者：
Park, Sung Young
中科院分区：
文献类型：
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作者：
Abdullah-Al-Nahain;Lee, Jung-Eun;Park, Sung Young
这项工作展示了使用透明质酸（HA） （GQD-HA）作为靶向剂实现石墨烯量子点（GQD）高效和靶向递送的方法。透明质酸被固定在GQD上，GQD接受与透明质酸共轭的儿茶酚部分盐酸多巴胺的迷人粘附特性，这一点经x射线光电子能谱证实。透射电子显微镜显示其粒径约为20 nm，荧光光谱显示在HA锚定后仍有明显的荧光强度。将制备的GQD-HA应用于CD44受体过表达的载瘤balb/c雌性小鼠，体内生物分布研究显示肿瘤组织荧光更加明亮。共聚焦激光扫描显微镜下的体外细胞成像显示，CD44过表达的A549细胞具有强烈的荧光。体内和体外实验结果均显示了透明质酸作为靶向分子的有效性。在弱酸性条件下，疏水药物阿霉素在GQD上的装载和释放动力学表明，GQD可以被认为是一种新型的药物载体，而MTT试验的无毒行为有力地支持了GQD- ha作为生物相容性材料的鉴定。
This work demonstrates the way to achieve efficient and target specific delivery of a graphene quantum dot (GQD) using hyaluronic acid (HA) (GQD-HA) as a targeting agent. HA has been anchored to a GQD that accepts the fascinating adhesive properties of the catechol moiety, dopamine hydrochloride, conjugated to HA, which was confirmed by X-ray photoelectron spectroscopy. Transmission electron microscopy revealed a particle size of similar to 20 nm, and the fluorescence spectra revealed significant fluorescence intensity even after the anchoring of HA. The prepared GQD-HA was applied to CD44 receptor overexpressed tumor-bearing balb/c female mice, and the in vivo biodistribution investigation demonstrated more bright fluorescence from the tumor tissue. In vitro cellular imaging, via a confocal laser scanning microscope, exhibited strong fluorescence from CD44 overexpressed A549 cells. Both in vivo and in vitro results showed the effectiveness of using HA as targeting molecule. The loading and release kinetics of the hydrophobic drug doxorubicin from a GQD under mildly acidic conditions showed that a GQD can be considered as a novel drug carrier, while the nontoxic behavior from the MTT assay strongly supports the identification of GQD-HA as a biocompatible material.