A Method for Phosphatase Assay in Tideland Soils

A Method for Phosphatase Assay in Tideland Soils
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潮汐土壤磷酸酶测定方法

DOI:
10.5985/jec.11.827
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发表时间:
2001
期刊:
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影响因子:
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通讯作者:
K. Hiraoka
K. Hiraoka
中科院分区:
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文献类型:
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作者:
Ken;C. Takaya;K. Hiraoka

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引言磷酸酶存在于所有土壤微生物中,催化有机磷酸化合物水解为无机磷酸盐。释放的磷酸盐对土壤微生物和植物至关重要,被认为是许多生态系统的限制因素1)2)。磷酸酶活性对许多环境扰动高度敏感,包括淹水3),肥料添加4)和重金属污染1)5)。此外,Neal报告说,土壤生物系统的变化会引起磷酸酶活性的扰动2)。从这些结果来看,磷酸酶已被接受为土壤质量的一个很好的潜在指标2,6,7)。在潮滩,沉积物中含有相对丰富的来自河流或海洋的有机物质。然而,只有少数出版物涉及海洋沉积物的酶活性8 -10)。磷酸盐是植物浮游生物、藻类和海藻生长所必需的。为了更好地了解海底生态系统,必须测量
INTRODUCTION Phosphatase is found in all soil microorganisms and catalyzes the hydrolysis of organic phosphate compounds into inorganic phosphate. The liberated phosphate is essential to soil microorganisms and plants and is considered to be a limiting factor in many ecosystems1)2). The phosphatase activity is highly sensitive to a number of environmental perturbations including waterlogging3), fertilizer additions4), and heavy metal pollution1)5). Moreover, Neal reported that changes in the soil biological system cause a perturbation in the phosphatase activity2). From these results, phosphatase has been accepted as a good potential indicator of soil quality2,6,7). In tidelands, sediments are relatively rich in organic substances supplied from rivers or the ocean. However, only a few publications have dealt with the enzymatic activity of marine sediments8-10). Phosphate is essential for the growth of plant planktons, algae and seaweed. To obtain a better insight into the benthic ecosystems, it is essential to measure