The power of real-time PCR
The power of real-time PCR
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DOI：
10.1152/advan.00019.2005
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发表时间：
2005-09-01
影响因子：
2.1
通讯作者：
Repa, JJ
中科院分区：
文献类型：
--
作者：
Valasek, MA;Repa, JJ
近年来，实时聚合酶链式反应(PCR)因其能够检测和定量极少量的特定核酸序列而成为一种强大而广泛应用于生物学研究的方法。作为一种研究工具，这项技术的一个主要应用是快速准确地评估由于生理学、病理生理学或发育而导致的基因表达变化。这种方法可以应用于模型系统，以测量对实验刺激的反应，并洞察蛋白质水平和功能的潜在变化。因此，生理学可以与分子事件相关联，以更好地了解生物过程。对于临床分子诊断，实时聚合酶链式反应可用于测量病毒或细菌载量或评估癌症状态。在这里，我们讨论了实时荧光定量聚合酶链式反应的基本概念、化学和仪器，并包括这项技术在生物医学科学和生命科学教育中的应用现状和前景。
In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene expression as a result of physiology, pathophysiology, or development. This method can be applied to model systems to measure responses to experimental stimuli and to gain insight into potential changes in protein level and function. Thus physiology can be correlated with molecular events to gain a better understanding of biological processes. For clinical molecular diagnostics, real- time PCR can be used to measure viral or bacterial loads or evaluate cancer status. Here, we discuss the basic concepts, chemistries, and instrumentation of real- time PCR and include present applications and future perspectives for this technology in biomedical sciences and in life science education.