Gene Banking for Ex Situ Conservation of Plant Genetic Resources
Gene Banking for Ex Situ Conservation of Plant Genetic Resources
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DOI：
10.1007/978-81-322-2283-5_23
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发表时间：
2015
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影响因子：
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通讯作者：
P. Rajasekharan
中科院分区：
文献类型：
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作者：
P. Rajasekharan
种子储存是最广泛和最有价值的保护方法之一。过去30年来，参与植物遗传资源的各机构和机构在这一领域发展了广泛的专门知识。种子库比其他保存情况的方法有相当大的优点，例如易于储存、节省空间、劳力需求相对较低，因此能够以经济上可行的成本保存大量样本。根据不同的品种，根据适当的规程将种子干燥到适当的低水分含量。通常情况下，这将小于5%。然后将种子储存在- 18°C或更低的温度下。由于种子DNA会随着时间的推移而降解，因此需要定期重新播种，并收集新鲜种子进行新一轮的长期储存。截至2006年，全世界大约有1300个基因库以种子的形式储存着大约600万份特定种群的样本。本章主要介绍了种子贮藏的方法及基因银行的最新研究进展。
Seed storage is one of the most widespread and valuableex situapproaches to conservation. Extensive expertise has been developed in this field by agencies and institutions involved with plant genetic resources over the past 30 years. Seed banking has considerable advantages over other methods ofex situconservation such as ease of storage, economy of space, relatively low labour demands and, consequently, the capacity to maintain large samples at an economically viable cost. Depending on the species, seeds are dried to suitably low moisture content according to an appropriate protocol. Typically this will be less than 5 %. The seeds then are stored at −18 °C or below. Because seed DNA degrades with time, the seeds need to be periodically replanted and fresh seeds collected for another round of long-term storage. There are about six million accessions, or samples of a particular population, stored as seeds in about 1,300 gene banks throughout the world as of 2006. The procedure for seed storage along with the latest development on gene banking is discussed in this chapter.