Preparation of a thiols β-cyclodextrin/gold nanoparticles-coated open tubular column for capillary electrochromatography enantioseparations
Preparation of a thiols β-cyclodextrin/gold nanoparticles-coated open tubular column for capillary electrochromatography enantioseparations
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DOI：
10.1002/jssc.201901323
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发表时间：
2020-04-13
影响因子：
3.1
通讯作者：
Jiang, Zhen
中科院分区：
文献类型：
--
作者：
Fang, Linlin;Zhao, Yanyan;Jiang, Zhen
受纳米粒子独特的化学和物理性质的启发，本文通过聚二烯丙基二甲基氯化铵静电组装到熔融石英毛细管内表面，然后自吸附带负电荷的SH-β-环糊精/金纳米粒子，制备了一种新型开管毛细管电色谱柱。通过扫描电子显微镜和能谱仪证实了SH-β-环糊精/金纳米粒子涂层毛细管的形成并对其进行了表征。扫描电镜和能谱分析结果表明，SH-β-环糊精/金纳米粒子成功地包覆在毛细管柱内壁上。通过对佐匹克隆、卡维地洛、沙丁胺醇、硫酸特布他林、盐酸酚苄明和布洛芬等6对手性药物的分析，验证了SH-β-环糊精/金纳米粒子涂层毛细管的性能。结果表明，纳米金作为载体可以提高开管毛细管柱的相对比。此外，还考察了SH-β-环糊精/纳米金包被毛细管柱的稳定性和重现性。该方法线性良好（>= 0.999），回收率为90.0 - 93.5%，重复性好，并成功地用于布洛芬对映体的分离，表明该柱在生物分析中具有潜在的应用价值。
Inspired by the distinct chemical and physical properties of nanoparticles, here a novel open-tubular capillary electrochromatography column was prepared by electrostatic assembly of poly(diallydimethylammonium chloride) onto the inner surface of a fused-silica capillary, followed by self-adsorption of negatively charged SH-beta-cyclodextrin/gold nanoparticles. The formation of the SH-beta-cyclodextrin/gold nanoparticles coated capillary was confirmed and characterized by scanning electron microscopy and energy dispersive spectrometry. The results of scanning electron microscopy and energy dispersive spectrometry studies indicated that SH-beta-cyclodextrin/gold nanoparticles were successfully coated on the inner wall of the capillary column. The performance of the SH-beta-cyclodextrin/gold nanoparticles coated capillary was validated by the analysis of six pairs of chiral drugs, namely zopiclone, carvedilol, salbutamol, terbutaline sulfate, phenoxybenzamine hydrochloride, and ibuprofen. Satisfactory enantioseparation results were achieved, confirming the use of gold nanoparticles as the support could enhance the phase ratio of the open-tubular capillary column. Additionally, the stability and reproducibility of the SH-beta-cyclodextrin/gold nanoparticles coated capillary column were also investigated. Then, this proposed method was well validated with good linearity (>= 0.999), recovery (90.0-93.5%) and repeatability, and was successfully used for enantioseparation of ibuprofen in spiked plasma samples, which indicated the new column's potential usage in biological analysis.