Gonadectomy and Hormone Replacement Affects In Vivo Basal Extracellular Dopamine Levels in the Prefrontal Cortex but Not Motor Cortex of Adult Male Rats
Gonadectomy and Hormone Replacement Affects In Vivo Basal Extracellular Dopamine Levels in the Prefrontal Cortex but Not Motor Cortex of Adult Male Rats
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DOI：
10.1093/cercor/bhq083
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发表时间：
2011-01-01
期刊：
影响因子：
3.7
通讯作者：
Kritzer, M. F.
中科院分区：
文献类型：
--
作者：
Aubele, T.;Kritzer, M. F.
已知成年雄性大鼠的性腺切除会损害多巴胺（DA）依赖性前额叶皮质任务的表现，并选择性地失调中前额叶DA系统的终点，包括轴突密度。在这项研究中，在体内微透析和高压液相色谱法被用来确定是否短期（4天）和/或长期（28天）性腺切除术和激素替代也可能影响更多的功能相关的度量基础细胞外DA水平/音。内侧前额叶皮质的评估显示，DA水平显着低于对照组在4天的性腺切除大鼠和类似于对照组在4天的性腺切除动物补充睾酮和雌二醇。在长期治疗组中，去性腺大鼠和给予雌二醇的去性腺大鼠的DA水平显著高于对照组，但给予睾酮的大鼠与对照组相似。与此相反，在运动皮层细胞外DA水平测量不受长期或短期性腺切除术。性腺切除术和激素替代对前额叶皮质DA水平的影响，在这里观察到的平行先前确定的影响，前额叶DA轴突密度，并可能代表激素的行动有关的DA依赖性前额叶皮质功能的调制，也许它的功能障碍，如精神分裂症，注意缺陷多动障碍，自闭症，男性受到不成比例的影响相对于女性。
Gonadectomy in adult male rats is known to impair performance on dopamine (DA)-dependent prefrontal cortical tasks and selectively dysregulate end points in the mesoprefrontal DA system including axon density. In this study, in vivo microdialysis and high-pressure liquid chromatography were used to determine whether short (4 day)- and/or long-term (28 day) gonadectomy and hormone replacement might also influence the more functionally relevant metric of basal extracellular DA level/tone. Assessments in medial prefrontal cortex revealed that DA levels were significantly lower than control in 4-day gonadectomized rats and similar to control in 4-day gonadectomized animals supplemented with both testosterone and estradiol. Among the long-term treatment groups, DA levels were significantly higher than control in gonadectomized rats and gonadectomized rats given estradiol but were similar to control in rats given testosterone. In contrast, extracellular DA levels measured in motor cortex were unaffected by long- or short-term gonadectomy. The effects of gonadectomy and hormone replacement on prefrontal cortical DA levels observed here parallel previously identified effects on prefrontal DA axon density and could represent hormone actions relevant to the modulation of DA-dependent prefrontal cortical function and perhaps its dysfunction in disorders such as schizophrenia, attention deficit hyperactivity disorder, and autism where males are disproportionately affected relative to females.