Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling
Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling
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骨桥蛋白通过 AKT 和 ERK1/2 信号促进人子宫内膜癌细胞 HEC-1A 的侵袭、迁移和上皮间质转化
DOI：
10.1159/000438518
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Kong, Ying
中科院分区：
文献类型：
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作者：
Li, Yinghua;Xie, Yunpeng;Kong, Ying
背景/目的：骨桥蛋白（OPN）是一种细胞外基质（ECM）分子，参与许多生理和病理过程，包括细胞黏附、血管生成和肿瘤转移。OPN是一种众所周知的多功能因子，涉及癌症进展的各个方面，包括子宫内膜癌。在本研究中，我们检测了OPN在子宫内膜癌中的意义。
方法：采用细胞计数试剂盒 - 8（CCK - 8）、划痕实验和Transwell实验检测HEC - 1A细胞的增殖、迁移和侵袭能力。采用蛋白质印迹法检测重组人骨桥蛋白（rhOPN）处理的HEC - 1A细胞中基质金属蛋白酶 - 2（MMP - 2）和上皮 - 间质转化（EMT）相关因子的表达。
结果：rhOPN促进HEC - 1A细胞的增殖、迁移和侵袭。rhOPN影响HEC - 1A细胞中EMT相关因子和MMP - 2的表达。rhOPN通过蛋白激酶B（PKB/AKT）和细胞外调节蛋白激酶（ERK1/2）信号通路促进HEC - 1A细胞的迁移、侵袭和上皮 - 间质转化。
结论：这些结果可能为子宫内膜癌开辟一种新的治疗策略：即rhOPN在控制子宫内膜癌细胞生长方面具有重要作用，并为这种癌症的治疗提出了一种新的靶向通路。（C）2015年，作者由巴塞尔的S. 卡尔格出版社出版
Background/Aims: Osteopontin (OPN) is an Extracellular Matrix (ECM) molecule and is involved in many physiologic and pathologic processes, including cell adhesion, angiogenesis and tumor metastasis. OPN is a well-known multifunctional factor involved in various aspects of cancer progression, including endometrial cancer. In this study, we examined the significance of OPN in endometrial cancer. Methods: The proliferation, migration and invasion ability of HEC-1A cells were detected by Cell Counting Kit-8 (CCK-8), Wound scratch assay and transwell. Western blots were employed to detect the expression of Matrix metalloproteinase-2 (MMP-2) and epithelial-mesenchymal transition (EMT)-related factors in HEC-1A cells treated with rhOPN. Results: rhOPN promotes cell proliferation, migration and invasion in HEC-1A cells. rhOPN influenced EMT-related factors and MMP-2 expression in HEC-1A cells. rhOPN promoted HEC-1A cells migration, invasion and EMT through protein kinase B (PKB/AKT) and Extracellular regulated protein kinases (ERK1/2) signaling pathway. Conclusions: These results may open up a novel therapeutic strategy for endometrial cancer: namely, rhOPN have important roles in controlling growth of endometrial of cancer cells and suggest a novel target pathway for treatment of this cancer.