Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby Canine Kidney cells
Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby Canine Kidney cells
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DOI：
10.1091/mbc.e05-08-0799
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发表时间：
2006-07-01
影响因子：
3.3
通讯作者：
Dunn, Kenneth W.
中科院分区：
文献类型：
--
作者：
Babbey, Clifford M.;Ahktar, Nahid;Dunn, Kenneth W.
Rab10是一种最初从马丁达比犬肾上皮细胞中分离出来的蛋白，属于Rab蛋白家族，包括Rab8和Rab13。虽然已经发现Rab8和Rab13都介导了极化的膜转运，但Rab10在哺乳动物细胞中的功能尚未确定。我们用表达野生型和突变型GFP嵌合体的极化MDCK细胞的定量共聚焦显微镜分析了Rab10在极化细胞中的功能。这些研究表明，Rab10特异性地与MDCK细胞的常见内吞体相关联，可被从顶端或基底侧质膜域内化的内吞探针所访问。突变的Rab10表达对GTP水解或GTP结合有缺陷，增加了从基底外侧早期隔间的再循环。内吞途径，而不影响从较后的隔室循环或顶端循环途径。这些结果表明，Rab10介导了从基侧分选内小体到普通内小体的转运。
Rab10, a protein originally isolated from Madin-Darby Canine Kidney (MDCK) epithelial cells, belongs to a family of Rab proteins that includes Rab8 and Rab13. Although both Rab8 and Rab13 have been found to mediate polarized membrane transport, the function of Rab10 in mammalian cells has not yet been established. We have used quantitative confocal microscopy of polarized MDCK cells expressing GFP chimeras of wild-type and mutant forms of Rab10 to analyze the function of Rab10 in polarized cells. These studies demonstrate that Rab10 is specifically associated with the common endosomes of MDCK cells, accessible to endocytic probes internalized from either the apical or basolateral plasma membrane domains. Expression of mutant Rab10 defective for either GTP hydrolysis or GTP binding increased recycling from early compartments on the basolateral. endocytic pathway without affecting recycling from later compartments or the apical recycling pathway. These results suggest that Rab10 mediates transport from basolateral sorting endosomes to common endosomes.