Aptamer based determination of Pb(II) by SERS and by exploiting the reduction of HAuCl4 by H2O2 as catalyzed by graphene oxide nanoribbons
Aptamer based determination of Pb(II) by SERS and by exploiting the reduction of HAuCl4 by H2O2 as catalyzed by graphene oxide nanoribbons
复制标题
基于适体的 Pb(II) 测定，采用 SERS 并利用氧化石墨烯纳米带催化的 H2O2 还原 HAuCl4
DOI：
10.1007/s00604-018-2714-9
复制
发表时间：
2018-03-01
影响因子：
5.7
通讯作者：
Jiang, Zhiliang
中科院分区：
文献类型：
--
作者：
Li, Chongning;Fan, Peidi;Jiang, Zhiliang
作者报道了氧化石墨烯纳米带对H2 O2还原HAuCl 4形成金纳米颗粒具有强烈的催化作用，金纳米颗粒显示出纳米等离子体表面增强拉曼散射（Sers）活性、瑞利散射和吸收。如果溶液中存在针对Pb（II）的适体，则其将结合到氧化石墨烯纳米带，从而抑制其催化活性。添加Pb（II）后，它将与适体结合形成稳定的复合物并释放自由的氧化石墨烯纳米带。这些导致在分子探针维多利亚蓝B存在下在1615 cm-1处的表面增强拉曼散射强度增加。在0.002-0.075 μmol·L-1铅浓度范围内，Sers信号呈线性增加，检测限为0.7 nmol·L-1。调色剂样品加标，然后通过该方法分析Pb（II）。相对标准偏差为6.2%~ 12.2%，回收率为86.7%~ 106.7%。基于Pb（II）与适体结合形成稳定的G-四链体并释放游离的氧化石墨烯纳米颗粒，以分子探针维多利亚蓝B为探针，建立了灵敏、选择性高的表面增强拉曼散射检测0. 002 ~ 0. 075 μmol. L-1 Pb（II）的方法。
The authors report that graphene oxide nanoribbons exert a strong catalytic effect on the reduction of HAuCl4by H2O2to form gold nanoparticles which display nanoplasmonic surface enhanced Raman scattering (SERS) activity, Rayleigh scattering and absorption. If an aptamer against Pb(II) is present in solution, it will bind to the graphene oxide nanoribbons and thereby inhibit their catalytic activity. Upon addition of Pb(II), it will bind to the aptamer to form stable complexes and release free graphene oxide nanoribbon. These cause the surface enhanced Raman scattering intensity at 1615 cm-1to increase in the presence of the molecular probe Victoria Blue B. The SERS signal increases linearly in the 0.002-0.075 μmol·L-1Pb(II) concentration range, and the detection limit is 0.7 nmol·L-1. Toner samples were spiked and then analyzed for Pb(II) by this method. Relative standard deviations are between 6.2% and 12.2%, and recoveries range from of 86.7%-106.7%. Graphic abstract Based on Pb(II) binds to the aptamer to form stable G-quadruplex and release free graphene oxide nanoribbon, a sensitive and selective surface enhanced Raman scattering method was developed for detection of 0.002-0.075 μmol·L-1Pb(II) by using the molecular probe Victoria Blue B.