Correlation between mercury concentration, and stable isotope ratios of carbon and nitrogen of amino acids in scalp hair from whale meat eaters and heavy fisheaters.
Correlation between mercury concentration, and stable isotope ratios of carbon and nitrogen of amino acids in scalp hair from whale meat eaters and heavy fisheaters.
复制标题
鲸肉食者和重食鱼者头皮毛发中汞浓度与氨基酸碳氮稳定同位素比之间的相关性。
DOI：
10.1002/rcm.7841
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
K,J.
中科院分区：
文献类型：
--
作者：
Endo;T.;Hayasaka;M.;Ogasawara;H.;Hotta;Y.;Kimura;O.;Petzke;K,J.
汞在海洋动物体内的积累一般会随着食物网中营养级（δ 15 N值）的增加而增加，捕食者积累的汞含量也会更高。人类摄入汞的主要来源是食用鱼类和其他海洋动物，头皮毛发中的汞浓度是评估海洋动物食用量的首选指标。方法采用同位素比值监测气相色谱/质谱联用技术，测定了大量食鱼者和食鲸者头皮毛发中15种氨基酸的δ 15 N值，并与其来源氨基酸的δ 15 N值进行比较用irm-GC/MS法测定了人发中汞的含量，并分析了人发中汞含量与各营养氨基酸δ 15 N值之间的相关性。（Ala、瓦尔、Leu、Ile、Pro、Asx和Glx）随汞浓度的增加而增加（p< 0.01），其中与Glx的相关性最高（R2= 0.725）。而Thr的δ 15 N值则随Hg浓度的增加而降低（R2= 0.663，p < 0.01）。Glx和Thr的δ 15 N值之差与汞浓度呈正相关，相关系数最高（R2= 0.773，p < 0.01）。版权所有© 2017约翰威利父子有限公司
RationaleAs mercury (Hg) accumulation in marine animals generally increases with increased trophic level (δ15N values) through the food web, predators accumulate higher levels of Hg. The main source of human Hg intake is the consumption of fish and other marine animals, and Hg concentration in scalp hair is the preferred marker for evaluating consumption of marine animals. Difference in δ15N values between trophic and source amino acids of human consumers could enable us to estimate the trophic level of the consumer without knowing the bulk δ15N value of their prey.MethodsWe measured the δ15N values of 15 amino acids in scalp hair from heavy fish eaters and whale meat eaters using isotope ratio monitoring gas chromatography/mass spectrometry (irm‐GC/MS), and investigated the correlations between Hg concentrations in the hair and the δ15N values of the individual constituent amino acids.ResultsThe δ15N values for all trophic amino acids (Ala, Val, Leu, Ile, Pro, Asx and Glx) increased with increases in Hg concentration (p< 0.01), with the highest correlation being with Glx (R2= 0.725). In contrast, the δ15N value for Thr decreased with increases in Hg concentration (R2= 0.663,p< 0.01). The difference in δ15N values between Glx and Thr was positively correlated with Hg concentration, showing the highest correlation coefficient (R2= 0.773,p< 0.01) among the various combinations for amino acids.ConclusionsThe difference in δ15N values between Glx and Thr appears to be the best proxy for the estimation of Hg concentration in scalp hair. Copyright © 2017 John Wiley & Sons, Ltd.