Evaluation of polyphenolic and flavonoid compounds in honeybee-collected pollen produced in Spain
Evaluation of polyphenolic and flavonoid compounds in honeybee-collected pollen produced in Spain
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DOI：
10.1021/jf0012300
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发表时间：
2001-04-01
影响因子：
6.1
通讯作者：
Lorente, EC
中科院分区：
文献类型：
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作者：
Bonvehí, JS;Torrentó, MS;Lorente, EC
测定了11份西班牙蜜蜂采集的花粉中多酚类化合物、类黄酮类化合物和游离类黄酮苷元化合物的含量。用乙酸乙酯对游离黄酮苷元进行了较好的提取。回收率(>；83.6%)，批内重复性(0.35 g/100 g，使用分光光度程序)。芦丁是游离类黄酮苷元类化合物的最佳鉴别物。建议芦丁的最低用量为200 mg/kg，以保证欧洲市场所需的营养和生物学特性。
Polyphenolic content, flavonoid content, and free flavonoid aglycon compounds were determined in 11 samples of Spanish honeybee-collected pollen. Adequate extraction was obtained with ethyl acetate in the determination of free flavonoid aglycon. Recovery (> 83.6%), within-run repeatability (0.35 g/100 g, using spectrophotometric procedures. Rutin is the best identifier of free flavonoid aglycon compounds. A minimum quantity of 200 mg/kg of rutin is suggested to guarantee the nutritional and biological properties required in the European market.