Environmental DNA as a tool to help inform zebra mussel, Dreissena polymorpha, management in inland lakes
Environmental DNA as a tool to help inform zebra mussel, Dreissena polymorpha, management in inland lakes
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DOI：
10.3391/mbi.2019.10.1.06
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发表时间：
2019-03-01
影响因子：
1.5
通讯作者：
Erickson, Richard A.
中科院分区：
文献类型：
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作者：
Amberg, Jon J.;Merkes, Christopher M.;Erickson, Richard A.
斑马贻贝（Dreissena polymorpha）是一种水生入侵物种，困扰着北美大部分地区，一旦它们建立起来就很难根除。因此，预防和监测是控制这些生物的关键要素。传统的显微镜通常用于监测，但需要幼虫面盘幼虫的存在。这就限制了资源管理员可以监测北方湖泊中存在的时间。一种新的监测工具，环境DNA（eDNA），可以允许一个更有效和更具成本效益的斑马贻贝监测计划。我们开发并测试了环境DNA检测在秋季和春季斑马贻贝在明尼苏达州的两个湖泊，一个严重出没，另一个新出没。我们发现，DNA拷贝数往往是较高的湖底附近和DNA更集中在较软的基板。我们还发现，斑马贻贝DNA的采样量在冬季导致类似的结果时，在秋季采样。这表明人们可以在冬季收集和分析斑马贻贝的eDNA，以帮助为未来的监测和控制工作提供信息。
Zebra mussels (Dreissena polymorpha) are an aquatic invasive species that plague much of North America and are difficult to impossible to eradicate once they become established. Therefore, prevention and monitoring are key elements in the control of these organisms. Traditional microscopy is commonly used in monitoring but requires the presence of larval veligers. This limits the times when resource managers can monitor for the presence in northern lakes. A new monitoring tool, environmental DNA (eDNA), may allow for a more efficient and cost-effective monitoring program for zebra mussels. We developed and tested an environmental DNA assay in the fall and spring for zebra mussels in two Minnesota lakes, one heavily infested and another newly infested. We found that DNA copy numbers tended to be higher near the lake bottom and DNA was more concentrated in softer substrates. We also found that the amount of zebra mussel DNA sampling in winter resulted in similar results to when sampled in fall. This suggests that one could collect and analyze eDNA for zebra mussels during winter months to help inform future efforts in monitoring and control.