miRNP:mRNA association in polyribosomes in a human neuronal cell line
miRNP:mRNA association in polyribosomes in a human neuronal cell line
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DOI：
10.1261/rna.5181104
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发表时间：
2004-03-01
期刊：
影响因子：
4.5
通讯作者：
Mourelatos, Z
中科院分区：
文献类型：
--
作者：
Nelson, PT;Hatzigeorgiou, AG;Mourelatos, Z
MicroRNA (miRNA) 是一种小型调节 RNA，通过与其 mRNA 靶标进行碱基配对来控制基因表达。 miRNA 组装成核糖核蛋白复合物，称为 miRNP。动物 miRNA 通过部分反义互补性识别其 mRNA 靶标，并在翻译起始后的一步抑制 mRNA 翻译。动物 miRNA 如何识别其 mRNA 靶标以及如何控制其翻译尚不清楚。在这里，我们描述了在人类神经元细胞系中，miRNP 蛋白 eIF2C2（Argonaute 蛋白家族的成员）、Gemin3 和 Gemin4 以及 miRNA 与多核糖体共沉积。此外，我们描述了含有let-7b (miRNA)的miRNP与其在含有多核糖体的级分中假定的人类mRNA靶标之间的物理关联。这些发现表明 miRNP 蛋白可能在靶标 mRNA 识别和翻译抑制中发挥重要作用。
MicroRNAs (miRNAs) are small regulatory RNAs that control gene expression by base-pairing with their mRNA targets. miRNAs assemble into ribonucleoprotein complexes termed miRNPs. Animal miRNAs recognize their mRNA targets via partial antisense complementarity and repress mRNA translation at a step after translation initiation. How animal miRNAs recognize their mRNA targets and how they control their translation is unknown. Here we describe that in a human neuronal cell line, the miRNP proteins eIF2C2 (a member of the Argonaute family of proteins), Gemin3, and Gemin4 along with miRNAs cosediment with polyribosomes. Furthermore, we describe a physical association between a let-7b (miRNA)-containing miRNP and its putative human mRNA target in polyribosome-containing fractions. These findings suggest that miRNP proteins may play important roles in target mRNA recognition and translational repression.