A Fluorescence Lifetime Distribution Measurement System Based on Phase-Resolved Detection Using an Image Dissector Tube
A Fluorescence Lifetime Distribution Measurement System Based on Phase-Resolved Detection Using an Image Dissector Tube
复制标题
基于图像解析管相位分辨检测的荧光寿命分布测量系统
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
S. Minami
中科院分区：
文献类型：
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作者：
X. F. Wang;T. Uchida;S. Minami
研制了一种新的基于析像管(IDT)的相分辨二维荧光测量系统。利用消隐电极的增益控制特性(位于IDT的开口板和第一达能极之间)，允许利用低电压控制信号进行外差检测。在IDT的消隐电极上对调制的荧光和参考信号进行互差，可提供高达1 GHz的高频响应，这与由于电子传递时间扩展而导致的动态节点链的20 MHz带宽形成了鲜明对比。观测到了时间分辨率的显著提高。荧光强度和寿命数据分别从相分辨信号的直流分量和交流分量获得。二维测量可以通过电子扫描IDT内的图像来执行。详细介绍了该仪器系统和典型的荧光寿命分布测量结果。
A new phase-resolved two-dimensional fluorescence measurement system using an image dissector tube (IDT) has been developed. The gain control characteristics of the blanking electrode (located between the aperture plate and the first dynode of the IDT) are utilized to allow heterodyne detection with low-voltage control signals. Heterodyning the modulated fluorescence and reference signals at the blanking electrode of the IDT provides high-frequency response up to 1 GHz, in contrast to the 20 MHz bandwidth of the dynode chain due to electron transit time spread. Significant improvement of time resolution is observed. Fluorescence intensity and lifetime data are derived from the dc and ac components of the phase-resolved signals, respectively. Two-dimensional measurements can be performed by electronically scanning images within the IDT. Details of the instrumental system and typical fluorescence lifetime distribution measurements are presented.
使用衰减时间分布对频域中测量的荧光衰减动力学进行分析。
影响因子：
3.8
作者：
Lakowicz,JR;Cherek,H;Gryczynski,I;Joshi,N;Johnson,ML
通讯作者：
Johnson,ML