Characterization of the altered cutaneous reactivity of transgenic mice whose keratinocytes overexpress B7-1.
Characterization of the altered cutaneous reactivity of transgenic mice whose keratinocytes overexpress B7-1.
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角质形成细胞过度表达 B7-1 的转基因小鼠皮肤反应性改变的表征。
DOI：
10.1006/clin.1997.4491
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
R. Barth
中科院分区：
文献类型：
--
作者：
A. Gaspari;R. Burns;S. Kondo;A. Nasir;A. Kurup;D. Mlodynia;D. Sauder;R. Barth
B7-1 （CD80）是第二个信号分子，通常与“专业”apc相关，可阻止t细胞克隆能量的诱导，并在抗原呈递过程中诱导IL-2的产生。表皮KC过表达B7-1的Tg小鼠对各种半抗原表现出夸张和持续的CHS，这种现象在半抗原刺激后持续长达8周。这些Tg小鼠对非持续性刺激物也表现出显著增强的耳肿胀反应。夸张的CHS未在引流淋巴结中反映。致敏和半抗原局部攻击后的t淋巴细胞增殖反应，因为B7-1 Tg和NTg小鼠之间没有显著差异。然而，在半抗原攻击部位对小鼠耳皮肤进行的RT-PCR分析表明，与NTg小鼠相比，B7-1 Tg小鼠的原位淋巴因子转录物动力学发生了改变：在2周时，在Tg小鼠皮肤中首次检测到ifn - γ转录物，而在NTg小鼠皮肤中则是24小时。检测半抗原应用位点炎症细胞因子转录物的RNase保护实验表明，与NTg小鼠相比，B7-1 Tg小鼠对半抗原应用有反应，tnf - α、IL-6和tnf - β转录物增加。因此，半抗原诱导的Tg小鼠耳肿胀可能在早期（1-14天）由炎症细胞因子的增强介导。产生ifn - γ的淋巴细胞可能与耳肿胀反应的晚期（14-42天）有关。这些数据表明，KC在小鼠皮肤中过表达B7-1直接或间接影响半抗原和刺激物诱导的皮肤炎症的性质。
B7-1 (CD80) is a second signal molecule usually associated with "professional" APCs that prevents the induction of T-cell clonal anergy and induces IL-2 production during antigen presentation. Tg mice whose epidermal KC overexpress B7-1 exhibit exaggerated and persistent CHS to a variety of haptens that lasts up to 8 weeks after hapten challenge. These Tg mice also exhibit significantly enhanced ear-swelling responses to irritants that are not persistent. Exaggerated CHS was not reflected in the draining lymph node. T-lymphocyte proliferative responses after sensitization and local challenge with haptens, as there were no significant differences between the B7-1 Tg and the NTg mice. However, RT-PCR analysis of mouse ear skin at the hapten challenge site indicated that B7-1 Tg mice had an alteration in the kinetics of in situ lymphokine transcripts compared to NTg mice: IFN-gamma transcripts were first detectable in Tg mouse skin at 2 weeks versus 24 h for NTg mice. RNase protection assays to detect inflammatory cytokine transcripts at hapten application sites indicated that B7-1 Tg mice responded to hapten application with increased TNF-alpha, IL-6, and TNF-beta transcripts compared to NTg mice. Thus, hapten-induced ear swelling in these Tg mice may be mediated by enhanced inflammatory cytokines during the early phase (1-14 days). IFN-gamma-producing lymphocytes may be responsible for the late phase of the ear-swelling response (14-42 days). These data indicate that B7-1 overexpression by KC in mouse skin directly or indirectly affects the nature of cutaneous inflammation induced by haptens and irritants.
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表皮角质形成细胞组成型表达 B7-1 抗原的转基因小鼠出现过度且持续的皮肤迟发型超敏反应。
DOI：
10.1172/jci117411
发表时间：
1994
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Nasir,A;Ferbel,B;Salminen,W;Barth,RK;Gaspari,AA
通讯作者：
Gaspari,AA
小鼠树突状表皮 T 细胞表达的细胞因子 mRNA 谱。
DOI：
10.1111/1523-1747.ep12365917
发表时间：
1993
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Matsue,H;CruzJr,PD;Bergstresser,PR;Takashima,A
通讯作者：
Takashima,A
转基因小鼠中 B7-1 的角质形成细胞表达增强了对皮肤抗原的初级免疫反应。
DOI：
10.1073/pnas.91.26.12780
发表时间：
1994
影响因子：
11.1
作者：
Williams,IR;Ort,RJ;Kupper,TS
通讯作者：
Kupper,TS
CD28 配体 B7 通过向 T 细胞提供共刺激信号来增强 IL-2 的产生。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Norton,SD;Zuckerman,L;Urdahl,KB;Shefner,R;Miller,J;Jenkins,MK
通讯作者：
Jenkins,MK
CTLA-4 受体存在与 B7 不同的额外配体的证据。
DOI：
10.1073/pnas.90.23.11182
发表时间：
1993
影响因子：
11.1
作者：
Razi-Wolf,Z;Galvin,F;Gray,G;Reiser,H
通讯作者：
Reiser,H