Thiol-Chromene "Click" Reaction-Activated Chemiluminescent Probe for Thiol Detection In Vitro and In Vivo.
Thiol-Chromene "Click" Reaction-Activated Chemiluminescent Probe for Thiol Detection In Vitro and In Vivo.
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DOI：
10.1021/acsami.3c05152
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发表时间：
2023-06
影响因子：
9.5
通讯作者：
Baoqu Wang;Xiaoxue Tian;Xing-Cong Li;K. Cheng;Min Xu
中科院分区：
文献类型：
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作者：
Baoqu Wang;Xiaoxue Tian;Xing-Cong Li;K. Cheng;Min Xu
与正常细胞相比，生物硫醇是在癌细胞中含量更高的生物分子，使其成为有用的癌症生物标志物。化学发光法因其灵敏度高、信噪比高而被广泛应用于生物成像领域。在这项研究中，我们设计并制备了一种荧光探针，它是由硫醇-色烯“点击”亲核反应激活的。该探针最初是荧光的，但在硫醇存在下关闭并释放极强的化学发光。与其他分析物相比，它对巯基具有很高的选择性。小鼠肿瘤部位的实时成像显示探针注射后有明显的化学发光，骨肉瘤组织的化学发光也明显强于癌旁组织。我们的结论是，这种荧光探针有可能检测硫醇，诊断癌症，特别是在其早期阶段，并有助于相关癌症药物的开发。
Biothiols are biomolecules found in a higher content in cancer cells compared to normal cells, marking them useful cancer biomarkers. Chemiluminescence is widely used in biological imaging due to its excellent sensitivity and signal-to-noise ratio (SNR). In this study, we designed and prepared a chemiluminescent probe that is activated by a thiol-chromene "click" nucleophilic reaction. This probe is initially chemiluminescent but turned off and releases extremely strong chemiluminescence in the presence of thiols. It has high selectivity to thiol compared with other analytes. Real-time imaging of mice tumor sites showed significant chemiluminescence after the probe was injected, and the chemiluminescence of osteosarcoma tissues was also significantly stronger than that in adjacent tissues. We conclude that this chemiluminescent probe has potential to detect thiol, diagnose cancer, especially in its early stages, and aid in the development of related cancer drugs.