PGC-1α is associated with C2C12 Myoblast differentiation
PGC-1α is associated with C2C12 Myoblast differentiation
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DOI：
10.2478/s11535-014-0341-y
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发表时间：
2014-08
影响因子：
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通讯作者：
Yaqiu Lin;Yanying Zhao;Ruiwen Li;J. Gong;Yucai Zheng;Yong Wang
中科院分区：
文献类型：
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作者：
Yaqiu Lin;Yanying Zhao;Ruiwen Li;J. Gong;Yucai Zheng;Yong Wang
PGC-1α是骨骼肌功能的重要调节因子。然而，PGC-1α在成肌细胞分化中的作用仍有待研究。在本研究中，我们观察到小鼠C2 C12成肌细胞分化过程中PGC-1α表达显著上调。为了解PGC-1α表达上调在成肌细胞分化中的生物学意义，分别用鼠PGC-1α cDNA和siRNA转染C2 C12细胞。PGC-1α过表达克隆融合形成典型的肌管，具有较高的肌球蛋白重链异构体I（MyHCI）mRNA水平和较低的MyHCIX。PGC-1α siRNA转染后，MyHCI和MyHCIIX mRNA水平明显下降，细胞分化不明显。此外，PGC-1α还能增加C2 C12细胞MyoD和MyoG的表达，从而控制前体细胞向肌管的定向分化。这些结果表明，PGC-1α与成肌细胞分化相关，并提高C2 C12细胞中MyoD和MyoG的表达水平。
PGC-1α has been implicated as an important mediator of functional capacity of skeletal muscle. However, the role of PGC-1α in myoblast differentiation remains unexplored. In the present study, we observed a significant up-regulation of PGC-1α expression during the differentiation of murine C2C12 myoblast. To understand the biological significance of PGC-1α up-regulation in myoblast differentiation, C2C12 cells were transfected with murine PGC-1α cDNA and siRNA targeting PGC-1α, respectively. PGC-1α over-expressing clones fused to form typical myotubes with higher mRNA level of myosin heavy chain isoform I (MyHCI) and lower MyHCIIX. No obvious differentiation was observed in PGC-1α-targeted siRNA-transfected cells with marked decrement of mRNA levels of MyHCI and MyHCIIX. Furthermore, PGC-1α increased the expression of MyoD and MyoG in C2C12 cells, which controlled the commitment of precursor cells to myotubes. These results indicate that PGC-1α is associated with myoblast differentiation and elevates MyoD and MyoG expression levels in C2C12 cells.