Adhesion and transcellular migration of neutrophils and B lymphocytes on fibroblasts
Adhesion and transcellular migration of neutrophils and B lymphocytes on fibroblasts
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DOI：
10.1016/j.yexcr.2009.04.013
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发表时间：
2009-08-01
影响因子：
3.7
通讯作者：
Thibault, Gaetan
中科院分区：
文献类型：
--
作者：
Couture, Patrick;Paradis-Massie, Jeremie;Thibault, Gaetan
在组织炎症过程中，浸润的白细胞可能与成纤维细胞发生物理接触。我们观察到，中性粒细胞和B淋巴细胞粘附在培养的成纤维细胞上的比例大于T细胞。显微镜检查显示粘附的特征还在于成纤维细胞吞噬白细胞。在迁移试验中，只有中性粒细胞和B淋巴细胞能够选择性地迁移通过成纤维细胞屏障。通过用肿瘤坏死因子-α（TNF-α）和佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）刺激，粘附和迁移增加。抗ICAM-1/β 2整联蛋白的抗体阻断了中性粒细胞与成纤维细胞的相互作用。对于B淋巴细胞，VCAM-1/α 4整合素对也参与这种相互作用。人皮肤成纤维细胞表现出与大鼠心脏成纤维细胞相似的粘附特征。通过测量迁移孔和钙粘蛋白阳性粘附连接的边界之间的距离，超过65%的孔对应于细胞旁途径的跨细胞途径。此外，波形蛋白染色显示，迁移孔高度嵌套的中间丝根据跨细胞路线。我们的结果表明，吞噬中性粒细胞和B淋巴细胞。成纤维细胞导致通过跨细胞途径的选择性传代。(C)2009 Elsevier Inc. All rights reserved.
During tissue inflammation, infiltrated leukocytes may have physical contacts with fibroblasts. We observed that neutrophils and B lymphocytes adhered in a larger proportion than T cells on cultured fibroblasts. Microscopy showed that adhesion was also characterized by leukocyte engulfment by the fibroblasts. In migration assays, only neutrophils and B lymphocytes were selectively able to migrate through a fibroblast barrier. Adhesion and migration were increased by stimulation with tumor necrosis factor-alpha (TNF-alpha) and phorbol-12-myristate-13-acetate (PMA). Antibodies against ICAM-1/beta 2 integrin blocked the interaction of neutrophils to fibroblasts. For B lymphocytes the couple VCAM-1/alpha 4 integrin was also involved in this interaction. Human skin fibroblasts presented similar adhesion characteristics as rat cardiac fibroblasts. By measuring the distance between the border of migration holes and cadherin-positive adherens junctions, more than 65% of the holes correspond to the transcellular route over the paracellular route. Furthermore, vimentin staining revealed that the migration holes were highly nested by intermediate filaments in accordance with the transcellular route. Our results demonstrated that engulfment of neutrophils and B lymphocytes by. fibroblasts resulted in selective passage by a transcellular route. (C) 2009 Elsevier Inc. All rights reserved.