A morphometric study of cytoskeletal reorganization in rat sciatic nerve axons following beta,beta'-iminodipropionitrile (IDPN) treatment.
A morphometric study of cytoskeletal reorganization in rat sciatic nerve axons following beta,beta'-iminodipropionitrile (IDPN) treatment.
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β,β-亚氨基二丙腈 (IDPN) 治疗后大鼠坐骨神经轴突细胞骨架重组的形态测量研究。
DOI：
10.1016/0304-3940(88)90596-4
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发表时间：
1988
影响因子：
2.5
通讯作者：
Lee,HY
中科院分区：
文献类型：
--
作者：
Nagele,RG;Bush,KT;Lee,HY
使用电子显微镜和形态测定法研究有髓鞘大鼠坐骨神经轴突中因暴露于 β,β'-亚氨基二丙腈 (IDPN) 而导致的细胞骨架元素（微管 (MT) 和神经丝 (NF)）组织的变化。成年雄性大鼠经神经膜下注射IDPN并每隔一段时间处死。注射神经节段薄片的电子显微照片显示，轴突中的 MT 与 NF 分离并形成明显的簇。对这些簇内微管间距离的形态测量表明，相邻 MT 具有相当均匀的空间分离，这似乎是由颗粒状 MT 相关的跨桥维持的。此外，在 IDPN 治疗的前 12 小时内，MT 簇经历了渐进但可逆的压实。
Electron microscopy and morphometry were used to study changes in the organization of cytoskeletal elements (microtubules (MTs) and neurofilaments (NFs)) in myelinated rat sciatic nerve axons resulting from exposure to β,β′-iminodipropionitrile (IDPN). Adult male rats were injected subepineurially with IDPN and sacrificed at intervals. Electron micrographs of thin sections through injected nerve segments revealed that MTs segregated from NFs in axons and formed prominent clusters. Morphometric measurements of intermicrotubular distances within these clusters showed that adjacent MTs had a fairly uniform spatial separation that appeared to be maintained by granular MT-associated cross-bridges. In addition, MT clusters underwent a progressive, but reversible, compaction during the first 12 h of IDPN treatment.