Detection of Bluetongue Virus RNA in Field-Collected Culicoides spp. (Diptera: Ceratopogonidae) Following the Discovery of Bluetongue Virus Serotype 1 in White-Tailed Deer and Cattle in Louisiana
Detection of Bluetongue Virus RNA in Field-Collected Culicoides spp. (Diptera: Ceratopogonidae) Following the Discovery of Bluetongue Virus Serotype 1 in White-Tailed Deer and Cattle in Louisiana
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DOI：
10.1603/me09211
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发表时间：
2010-03-01
影响因子：
2.1
通讯作者：
Foil, L. D.
中科院分区：
文献类型：
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作者：
Becker, M. E.;Reeves, W. K.;Foil, L. D.
2004年11月，在美国洛杉矶圣玛丽教区的一只被猎杀的鹿身上首次发现蓝舌病病毒（呼肠孤病毒科，奥比病毒属，BTV）血清型1 （BTV-1）。2005年，对发现鹿的地区附近的3个牛场进行了血清调查，其中2个牛场发现btv -1血清阳性的牛；2006年，对这三个农场的牛的血清调查未发现任何btv -1阳性动物。本研究的目的是调查三个养殖场的蠓种群和试验场采集的BTV和流行性出血病病毒（呼肠孤病毒科，奥比病毒属，EHDV）的存在情况。2006年1月至2007年11月，在三个养殖场采用小型CDC灯诱和新泽西捕蝇器捕获蠓油。共捕获蠓3319只，其中库蠓10种1790只。采用ir - rt聚合酶链反应（PCR）对在养殖场采集的2,309份标本的264个池进行BTV和EHDV筛查。所有阳性样本测序测定血清型。5组275例（1.8%）BTV阳性。阳性库蠓有4种库蠓，分别为黄昏库蠓（Malloch）、褐皮库蠓（2个库）、马洛赫血红库蠓（Malloch）和富伦库蠓（Poey）。阳性标本的扩增子序列与BTV-17和BTV-13相同。在我们的研究中，未检测到BTV-1在牛中传播，在蠓类标本中未检测到BTV-1。
In November 2004, bluetongue virus (family Reoviridae, genus Orbivirus, BTV) serotype 1 (BTV-1) was detected for the first time in the United States from a hunter-killed deer in St. Mary Parish, LA. In 2005, sera surveys were conducted on three cattle farms near the area where the deer was found, and BTV-1-seropositive cattle were found oil two of the three farms; in 2006, sera surveys from the cattle on the three farms did not detect any BTV-1-positive animals. The purpose of this study was to survey ceratopogonid populations at the three farms and test field-collected specimens for the presence of BTV and epizootic hemorrhagic disease virus (family Reoviridae, genus Orbivirus, EHDV). Miniature CDC light traps and New Jersey traps were used to capture ceratopogonids oil the three farms from January 2006 through November 2007. In total, 3,319 ceratopogonids were captured, including 1,790 specimens of 10 different species of Culicoides. IR-RT-polymerase chain reaction (PCR) was performed to screen for BTV and EHDV in 264 pools representing 2,309 specimens collected at the farms. All positive samples were sequenced for serotype determination. Five pools of 275 (1.8%) were positive for BTV. Pools of four species of Culicoides were found to be positive: Culicoides crepuscularis (Malloch), Culicoides debilipalpis Lutz (two pools), Culicoides haematopotus Malloch, and Culicoides furens (Poey). The amplicons of the positive specimens were sequenced and found to be identical to both BTV-17 and BTV-13. During our study, no BTV-1 transmission was detected in cattle, and no BTV-1 was detected in specimens of ceratopogonids.