Optimising the extraction of tea polyphenols, (−)‐epigallocatechin gallate and theanine from summer green tea by using response surface methodology
Optimising the extraction of tea polyphenols, (−)‐epigallocatechin gallate and theanine from summer green tea by using response surface methodology
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DOI：
10.1111/j.1365-2621.2012.03082.x
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发表时间：
2012-10
影响因子：
3.3
通讯作者：
Xin Zhang;Fei Xu;Yuan Gao;Jinghe Wu;Yi Sun;Xiaoxiong Zeng
中科院分区：
文献类型：
--
作者：
Xin Zhang;Fei Xu;Yuan Gao;Jinghe Wu;Yi Sun;Xiaoxiong Zeng
本研究采用响应面分析法对夏绿色茶中茶多酚、表没食子儿茶素没食子酸酯（EGCG）和茶氨酸的提取工艺进行了优化。结果表明，最佳提取条件为温度96 °C，时间40 min，料水比16 mL g−1。在此条件下，茶多酚、没食子酸（EGCG）、茶氨酸和DPPH自由基清除IC 50分别为133.41 ± 3.12 mg g−1、30.23 ± 1.22、3.99 ± 1.13 mg g−1和724.95 ± 15.12 μg mL−1。在0.05的显著水平下，实验值与预测值之间无统计学差异。结果表明，所建立的回归模型可用于夏季绿色茶生物活性物质的提取。
Summary In this study, response surface methodology was applied to determine the optimal conditions for the extraction of tea polyphenols, EGCG and theanine from summer green tea. As results, the optimal extraction conditions were determined to be temperature 96 °C, time 40 min and ratio of water to raw material 16 mL g−1. Under these optimal conditions, the experimental values for tea polyphenols, EGCG, theanine and IC50 for DPPH free radical scavenging activity were 133.41 ± 3.12 mg gallic acid g−1, 30.23 ± 1.22, 3.99 ± 1.13 mg g−1 and 724.95 ± 15.12 μg mL−1, respectively. There was no statistical difference at significant level of 0.05 between the experimental and predicted values. The results suggested that the regression models were accurate and adequate for the bioactives extraction from summer green tea.