Effect of experimental liver disease on the utilization for protein synthesis of orally administered alpha-ketoisocaproate.
Effect of experimental liver disease on the utilization for protein synthesis of orally administered alpha-ketoisocaproate.
复制标题
实验性肝病对口服α-酮异己酸蛋白质合成利用的影响。
DOI：
10.1002/hep.1840060325
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Walser,M
中科院分区：
文献类型：
--
作者：
Muñoz,S;Walser,M
The incorporation of orally administered 1-14 C-α-ketoisocaproate into the leucine of proteins in rats was compared with the incorporation of [3 H] leucine itself administered simultaneously and expressed as a ratio, R. This ratio in whole body protein has been shown to be approximately equal to the nutritional efficiency of α-ketoisocaproate as a dietary substitute for leucine. In normal rats on a 14% protein diet, R in whole body protein (0.30±0.01) and in the protein of various organs was the same whether the isotopes were given by single injection or 6-hr constant infusion. Thus, both techniques yield the same time-independent parameter, R, which measures the relative efficiency of α-ketoisocaproate as a substitute for leucine. R varied between organs as follows: liver (0.22±0.01)< kidney< heart< salivary gland< brain< muscle (0.42±0.01). In rats with galactosamine-induced acute liver failure (Group I), carbon tetrachloride-induced cirrhosis (Group II), or portal-systemic shunts (Group III), whole body protein R and R in the protein of organs other than the liver was generally increased compared with controls, as was R in circulating IgG in Group III; R in liver protein was unchanged (Groups II and III) or slightly lower than controls (Group I). Thus, severe liver disease and portal-systemic shunting both increase the utilization of α-ketoisocaproate for synthesis of protein in the body as a whole and in most organs. In the liver, however, α-ketoisocaproate utilization for protein synthesis is unaffected or slightly reduced.
