Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer.
Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer.
复制标题
通过 DNA 介导的基因转移鉴定 BALB/c H-2Ld 基因。
作者：
R. Goodenow;M. Mcmillan;A. Orn;M. Nicolson;N. Davidson;J. Frelinger;L. Hood
基因转移和免疫选择用于鉴定含有H-2Ld基因的BALB/c基因组克隆（克隆27.5）。用克隆的27.5 DNA与单纯疱疹病毒tk基因一起转化胸苷激酶阴性的C3 H小鼠L细胞，产生表达Ld分子的转化子，所述转化子通过用针对Ld抗原的单克隆杂交瘤抗体的放射免疫测定来检测。通过H-2Ld免疫沉淀物的二维电泳分析，克隆小鼠L细胞转化体表达的外源Ld基因产物与BALB/c脾Ld分子几乎没有区别。这些结果表明，基因组克隆27.5包含一个功能BALB/c H-2Ld基因，并证明了这种方法的有用性，用于鉴定由克隆的基因编码的基因产物，这些基因是一个多基因家族的成员。此外，将细胞表面识别分子置于外源细胞表面的能力为这些分子的功能分析提供了强大的机会。
Gene transfer and immunoselection were used in the identification of a BALB/c genomic clone containing an H-2Ld gene (clone 27.5). Transformation of thymidine kinase-negative C3H mouse L cells with the cloned 27.5 DNA together with the herpes simplex virus tk gene produced transformants expressing Ld molecules detected by radioimmune assay with monoclonal hybridoma antibodies to Ld antigens. The foreign Ld gene products expressed by cloned mouse L cell transformants were shown to be virtually indistinguishable from BALB/c spleen Ld molecules by two-dimensional electrophoretic analysis of H-2Ld immunoprecipitates. These results indicate that the genomic clone 27.5 contains a functional BALB/c H-2Ld gene and demonstrate the usefulness of this approach for identifying the gene products encoded by cloned genes which are members of a multigene family. Furthermore, the ability to place cell-surface recognition molecules on the surfaces of foreign cells provides a powerful opportunity for functional analyses of these molecules.
编码 BALB/c 小鼠 Ld 移植抗原的基因的 DNA 序列。
DOI：
10.1126/science.7058332
发表时间：
1982
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Moore,KW;Sher,BT;Sun,YH;Eakle,KA;Hood,L
通讯作者：
Hood,L