Microbial communities inhabiting oil-contaminated soils from two major oilfields in Northern China: Implications for active petroleum-degrading capacity
Microbial communities inhabiting oil-contaminated soils from two major oilfields in Northern China: Implications for active petroleum-degrading capacity
复制标题
DOI：
10.1007/s12275-015-5023-6
复制
发表时间：
2015-05
影响因子：
3
通讯作者：
Weimin Sun;Yiran Dong;Pin Gao;Meiyan Fu;K. Ta;Jiwei Li
中科院分区：
文献类型：
--
作者：
Weimin Sun;Yiran Dong;Pin Gao;Meiyan Fu;K. Ta;Jiwei Li
尽管油田中存在着多种具有不同代谢潜力的微生物，但由于绝大多数石油降解细菌尚未培养，因此我们目前对石油降解细菌的了解有限。本研究采用16S rRNA基因高通量测序技术，对大庆和长庆油田9个石油污染土壤中的微生物群落进行了分析。其中变形菌门（Proteobacteria）占优势，放线菌门（Actinobacteria）占优势。在属级水平上，在9个土壤样品中检出较多的有链烷酶属、节杆菌属、假单胞菌属、分枝杆菌属和红球菌属。许多优势属与已知的石油降解物种有亲缘关系。还研究了微生物群落内理化参数之间的相关性。典范对应分析表明，土壤水分，硝酸盐，TOC和pH值有重要的影响，在塑造碳氢化合物污染土壤的微生物群落。本研究为石油污染土壤微生物群落的深入分析和石油污染的生物修复提供了有益的信息。
Although oilfields harbor a wide diversity of microorganisms with various metabolic potentials, our current knowledge about oil-degrading bacteria is limited because the vast majority of oil-degrading bacteria remain uncultured. In the present study, microbial communities in nine oil-contaminated soils collected from Daqing and Changqing, two of the largest oil fields in China, were characterized through highthroughput sequencing of 16S rRNA genes. Bacteria related to the phylaProteobacteriaandActinobacteriawere dominant in four and three samples, respectively. At the genus level,Alkanindiges,Arthrobacter,Pseudomonas,Mycobacterium, andRhodococcuswere frequently detected in nine soil samples. Many of the dominant genera were phylogenetically related to the known oil-degrading species. The correlation between physiochemical parameters within the microbial communities was also investigated. Canonical correspondence analysis revealed that soil moisture, nitrate, TOC, and pH had an important impact in shaping the microbial communities of the hydrocarbon-contaminated soil. This study provided an in-depth analysis of microbial communities in oilcontaminated soil and useful information for future bioremediation of oil contamination.