Visualizing the 3D cytoarchitecture of the human cochlea in an intact temporal bone using synchrotron radiation phase contrast imaging
Visualizing the 3D cytoarchitecture of the human cochlea in an intact temporal bone using synchrotron radiation phase contrast imaging
复制标题
DOI：
10.1364/boe.9.003757
复制
发表时间：
2018-08-01
影响因子：
3.4
通讯作者：
Stankovic, Konstantina M.
中科院分区：
文献类型：
--
作者：
Iyer, Janani S.;Zhu, Ning;Stankovic, Konstantina M.
可视化人类感音神经性听力损失病理的金标准方法125年来一直是死后组织学，尽管人们意识到组织学准备会在生物组织中诱发严重的伪影。从历史上看，从死后评估到非侵入性临床生物医学成像的转变已经彻底改变了疾病的诊断和治疗；然而，由于耳蜗体积小、三维结构复杂、易碎且深嵌于骨内，人类耳蜗细胞水平成像的非侵入性技术的创新一直很困难。现在，我们研究了同步辐射促进的x射线吸收和相衬成像的能力，以便在3D完整、未脱钙、未染色的人类颞骨中原位显示耳蜗感觉上皮中的感觉细胞和神经纤维。我们的研究结果表明，这种成像技术以前所未有的细胞细节水平解决了骨包膜感觉上皮的细胞结构，对于一个完整的，未染色的标本，并且能够区分健康和受损的上皮。所有的分析都是使用商用软件进行的，该软件可以快速重建并促进大量数据集的3D操作。结果表明，同步辐射相位对比成像在未来有可能取代组织学作为评估人类标本耳蜗内结构完整性的金标准，并促进进一步优化以转化为临床。(C) 2018年美国光学学会根据OSA开放获取出版协议的条款
The gold standard method for visualizing the pathologies underlying human sensorineural hearing loss has remained post-mortem histology for over 125 years, despite awareness that histological preparation induces severe artifacts in biological tissue. Historically, the transition from post-mortem assessment to non-invasive clinical biomedical imaging in living humans has revolutionized diagnosis and treatment of disease; however, innovation in non-invasive techniques for cellular-level intracochlear imaging in humans has been difficult due to the cochlea's small size, complex 3D configuration, fragility, and deep encasement within bone. Hem we investigate the ability of synchrotron radiation-facilitated X-ray absorption and phase contrast imaging to enable visualization of sensory cells and nerve fibers in the cochlea's sensory epithelium in situ in 3D intact, non-decalcified, unstained human temporal bones. Our findings show that this imaging technique resolves the bone-encased sensory epithelium's cytoarchitecture with unprecedented levels of cellular detail for an intact, unstained specimen, and is capable of distinguishing between healthy and damaged epithelium. All analyses were performed using commercially available software that quickly reconstructs and facilitates 3D manipulation of massive data sets. Results suggest that synchrotron radiation phase contrast imaging has the future potential to replace histology as a gold standard for evaluating intracochlear structural integrity in human specimens, and motivate further optimization for translation to the clinic. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement