Differential expression of thromboxane A synthase and prostaglandin H synthase in megakaryocytic cell line.
Differential expression of thromboxane A synthase and prostaglandin H synthase in megakaryocytic cell line.
复制标题
巨核细胞系中血栓素 A 合酶和前列腺素 H 合酶的差异表达。
DOI：
10.1016/0167-4889(95)00116-a
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Wu,KK
中科院分区：
文献类型：
--
作者：
Matijevic-Aleksic,N;Sanduja,SK;Wang,LH;Wu,KK
我们测定了人巨核细胞系（MEG-01）中前列腺素H合酶（PGHS）和血栓素A合酶（TXAS）亚型的表达。与未诱导细胞中的PGHS-1和PGHS-2相比，全长TXAS mRNA和60 kDa TXAS蛋白的基础水平较高。尽管TXAS水平较高，但由于PGHS-1或PGHS-2表达有限，未诱导的MEG-01细胞仅合成少量血栓烷A2（TXA 2）。PMA诱导后TXAS变化不大。PMA处理3 h后，PGHS-2 mRNA水平显著升高，随后迅速下降，而PGHS-1 mRNA和蛋白水平则一致升高。PGHS-1的诱导在PMA处理的第3天达到平台期。对PMA诱导3天和5天的细胞中花生四烯酸代谢的分析表明，PGH_2合成水平高，超过了TXAS合成TXA_2的能力。在人血小板中仅检测到痕量的PGHS-2 mRNA和选择性剪接的TXAS mRNA。我们得出结论，TXAS和PGHS在PMA诱导分化过程中MEG-01的差异表达。
We determined the expression of isoforms of prostaglandin H synthase (PGHS) and thromboxane A synthase (TXAS) in a human megakaryocyte cell line (MEG-01. The basal levels of full-length TXAS mRNA and the 60 kDa TXAS protein were high when compared to those of PGHS-1 and PGHS-2 in uninduced cells. Despite a high TXAS level, uninduced MEG-01 cells synthesized only a small amount of thromboxane A2(TXA2) due to limited PGHS-1 or PGHS-2 expressions. Following PMA induction there was little change in TXAS. PGHS-2 mRNA was significantly increased at only 3 h of PMA treatment and the level declined rapidly, whereas PGHS-1 mRNA and protein levels were concordantly stimulated. Induction of PGHS-1 reached plateau on day 3 of PMA treatment. Analysis of arachidonate metabolism in cells induced by PMA for 3 and 5 days showed a high level of PGH2synthesis which exceeded the TXAS capacity for TXA2synthesis. Only traces of PGHS-2 mRNA and alternate-spliced TXAS mRNA were detected in human platelets. We conclude that TXAS and PGHS are differentially expressed in MEG-01 during PMA-induced differentiation.