NEW FLUORESCENT VIABILITY TEST FOR FUNGI CELLS
NEW FLUORESCENT VIABILITY TEST FOR FUNGI CELLS
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DOI：
10.1007/bf00683967
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发表时间：
1979-01-01
期刊：
影响因子：
5.5
通讯作者：
PAULA, CR
中科院分区：
文献类型：
--
作者：
CALICH, VLG;PURCHIO, A;PAULA, CR
荧光素二乙酸酯-溴化乙锭(FDA-EB)荧光法主要用于测定哺乳动物细胞的活力，应用于几种真菌。活的真菌细胞产生荧光染色，即细胞内荧光素的积累，在紫外光显微镜下很容易看到绿色。死细胞因溴化乙锭渗入而呈鲜红色。FDA-EB试验与菌落计数法有很好的相关性，可以成功地用于酵母和酵母样细胞活性的测定。FDA-EB试验的主要优点是快速、灵敏度高、操作简便。
The fluorescein diacetate-ethidium bromide (FDA-EB) fluorescence method, primarily used to determine viability of mammalian cells, was applied to several fungi species. Living fungi cells produced fluorochromasia, i.e., an intracellular accumulation of fluorescein which could be easily visualized as a green color under the U.V. microscope. Dead cells showed a red bright color due to ethidium bromide penetration. The FDA-EB test can be successfuly employed to assay yeast and yeast like cells viability since a good correlation was observed between this assay and the colony count technique. The main advantages of FDA-EB test are its speed, high sensitivity and simplicity.