Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana.
Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana.
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表型筛选和全基因组重测序揭示的γ射线诱导拟南芥突变的频率和谱。
DOI：
10.3390/ijms23020654
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发表时间：
2022-01-07
影响因子：
5.6
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Du Y;Feng Z;Wang J;Jin W;Wang Z;Guo T;Chen Y;Feng H;Yu L;Li W;Zhou L
遗传变异是种质多样性的重要来源，因为它为植物育种提供了有助于开发新性状的等位基因资源。γ射线作为一种物理诱变剂已被广泛应用于植物诱变育种，其诱变效应引起了人们的广泛关注。然而，在大规模的表型突变筛查和全基因组突变扫描中，很少有全面的突变谱研究。本研究通过M1代生物学效应、M2代大规模表型筛选以及7个M3表型可见株系的全基因组重测序，综合评价了γ射线对拟南芥的诱变效应。从20，502株M2植株中共分离到417株具有可见突变表型的植株，γ射线在拟南芥中的表型突变频率为2.03%。平均而言，每个品系中有21.57个单碱基替换（SBS）和11.57个小插入和缺失（InDel）。单碱基InDel占小InDel的66.7%。基因组突变频率为2.78 × 10−10/bp/戈伊。转换/颠换比率为1.60，64.28%的C > T事件显示嘧啶二核苷酸序列; 69.14%的小InDel位于用于DNA末端重接的具有1 - 4 bp末端微同源性的序列中，而SBS较少依赖于末端微同源性。在每个重新测序的品系中，平均有9个基因被预测会发生功能改变。这表明，在对某一性状或少数性状进行优良材料改良时，适宜的诱变基因密度是γ射线的优势。这些结果将有助于全面了解γ射线的诱变效应和机理，并为合适的诱变剂筛选和参数设计提供依据，从而进一步促进植物突变育种中更可控的诱变方法的发展。
Genetic variations are an important source of germplasm diversity, as it provides an allele resource that contributes to the development of new traits for plant breeding. Gamma rays have been widely used as a physical agent for mutation creation in plants, and their mutagenic effect has attracted extensive attention. However, few studies are available on the comprehensive mutation profile at both the large-scale phenotype mutation screening and whole-genome mutation scanning. In this study, biological effects on M1 generation, large-scale phenotype screening in M2 generation, as well as whole-genome re-sequencing of seven M3 phenotype-visible lines were carried out to comprehensively evaluate the mutagenic effects of gamma rays on Arabidopsis thaliana. A total of 417 plants with visible mutated phenotypes were isolated from 20,502 M2 plants, and the phenotypic mutation frequency of gamma rays was 2.03% in Arabidopsis thaliana. On average, there were 21.57 single-base substitutions (SBSs) and 11.57 small insertions and deletions (InDels) in each line. Single-base InDels accounts for 66.7% of the small InDels. The genomic mutation frequency was 2.78 × 10−10/bp/Gy. The ratio of transition/transversion was 1.60, and 64.28% of the C > T events exhibited the pyrimidine dinucleotide sequence; 69.14% of the small InDels were located in the sequence with 1 to 4 bp terminal microhomology that was used for DNA end rejoining, while SBSs were less dependent on terminal microhomology. Nine genes, on average, were predicted to suffer from functional alteration in each re-sequenced line. This indicated that a suitable mutation gene density was an advantage of gamma rays when trying to improve elite materials for one certain or a few traits. These results will aid the full understanding of the mutagenic effects and mechanisms of gamma rays and provide a basis for suitable mutagen selection and parameter design, which can further facilitate the development of more controlled mutagenesis methods for plant mutation breeding.
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影响因子：
7.2
作者：
Hirano, Tomonari;Kazama, Yusuke;Abe, Tomoko
通讯作者：
Abe, Tomoko
影响因子：
13.8
作者：
Datta S;Jankowicz-Cieslak J;Nielen S;Ingelbrecht I;Till BJ
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Till BJ
拟南芥中碳离子束照射诱导的取代和小插入缺失的鉴定。
影响因子：
5.6
作者：
Du Y;Luo S;Li X;Yang J;Cui T;Li W;Yu L;Feng H;Chen Y;Mu J;Chen X;Shu Q;Guo T;Luo W;Zhou L
通讯作者：
Zhou L
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
1.1
作者：
Ishii, Kotaro;Kazama, Yusuke;Abe, Tomoko
通讯作者：
Abe, Tomoko