Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line
Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line
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DOI：
10.1007/s00441-010-1037-x
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发表时间：
2010-11-01
影响因子：
3.6
通讯作者：
Akamine, Akifumi
中科院分区：
文献类型：
--
作者：
Fujii, Shinsuke;Maeda, Hidefumi;Akamine, Akifumi
牙周膜（PDL）是一种特殊的结缔组织，影响牙齿的寿命。转化生长因子-β 1（Transforming growth factor-beta 1，TGF-β 1）是一种多功能细胞因子，但其对PDL细胞的影响尚不清楚。我们的目的是证明TGF-β 1在大鼠PDL组织中的表达，并评估其对人PDL细胞（HPLCs）和我们最近开发的人PDL干/祖细胞系1-11中增殖和基因表达的影响。免疫化学证实了整个PDL组织中TGF-β 1的表达，HPLC和细胞系1-11均表达TGF-β 1、TGF-β I型受体和TGF-β II型受体基因的mRNA。虽然外源性TGF-β 1刺激了HPLC的增殖，但它并不上调HPLC中α-平滑肌肌动蛋白（α-SMA）、I型胶原（Col I）或α-SMA蛋白或α-平滑肌肌动蛋白-1（FBN 1）mRNA的表达，而这些基因的表达被抗TGF-β 1中和抗体减弱。相反，外源性TGF-β 1降低了细胞系1-11的增殖，尽管它上调了该细胞系中α-SMA、Coil和FBN 1 mRNA以及α-SMA蛋白的表达。此外，白细胞介素-1 β刺激显着降低HPLC中TGF-β 1 mRNA和蛋白的表达。因此，TGF-β 1似乎在诱导PDL干/祖细胞的成纤维细胞分化和在生理条件下维持PDL装置中起重要作用。
Periodontal ligament (PDL) is a specialized connective tissue that influences the lifespan of the tooth. Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional cytokine, but little is known about the effects of TGF-beta 1 on PDL cells. Our aim has been to demonstrate the expression of TGF-beta 1 in rat PDL tissues and to evaluate its effects on the proliferation and gene expression in human PDL cells (HPLCs) and a human PDL stem/progenitor cell line, line 1-11, that we have recently developed. The expression of TGF-beta 1 in the entire PDL tissue was confirmed immunohistochemically, and both HPLCs and cell line 1-11 expressed mRNA from the TGF-beta 1, TGF-beta type I receptor, and TGF-beta type II receptor genes. Although exogenous TGF-beta 1 stimulated the proliferation of HPLCs, it did not upregulate the expression of alpha-smooth muscle actin (alpha-SMA), type I collagen (Col I), or fibrillin-1 (FBN 1) mRNA or of a-SMA protein in HPLCs, whereas expression for these genes was attenuated by an anti-TGF-beta 1 neutralizing antibody. In contrast, exogenous TGF-beta 1 reduced the proliferation of cell line 1-11, although it upregulated the expression of alpha-SMA, Coil, and FBN 1 mRNA and of alpha-SMA protein in this cell line. In addition, interleukin-1 beta stimulation significantly reduced the expression of TGF-beta 1 mRNA and protein in HPLCs. Thus, TGF-beta 1 seems to play an important role in inducing fibroblastic differentiation of PDL stem/progenitor cells and in maintaining the PDL apparatus under physiological conditions.