Role of vascular angiotensin II released by beta-adrenergic stimulation in rats.
Role of vascular angiotensin II released by beta-adrenergic stimulation in rats.
复制标题
β-肾上腺素能刺激释放的血管血管紧张素 II 在大鼠中的作用。
DOI：
10.1097/00005344-198600101-00002
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发表时间：
1986
影响因子：
3
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Nakamaru,M;Jackson,EK;Inagami,T
在体外检测了[β]-肾上腺素受体激动剂对血管肾素-血管紧张素系统组分释放的影响。在开放系统中用Krebs-Ringer溶液灌注离体大鼠肠系膜动脉，并使用连接到灌注系统的Sep-Pak C-18柱直接测定释放到灌注液中的免疫反应性血管紧张素II（ANG IIir）。还测定了浓缩灌流液中的肾素活性。异丙肾上腺素（1 nM-1 [mu] M）以剂量依赖性方式增加ANG IIir的释放。在异丙肾上腺素（1 [mu] M）输注过程中ANG IIir释放的增加被普萘洛尔（1 [mu] M）或卡托普利（2 [mu] M）抑制。异丙肾上腺素诱导的ANG IIir释放增加被选择性[β] 2-肾上腺素受体拮抗剂ICI 118，551（1 [mu] M）阻断，但不被选择性[β] 1-肾上腺素受体拮抗剂阿替洛尔（1 [mu] M）阻断。灌注液中的肾素活性是可测量的，但对异丙肾上腺素（1 [mu] M）输注没有反应。自发性高血压大鼠和Wistar-Kyoto大鼠的ANG IIir释放对异丙肾上腺素的反应无显著差异。目前的结果表明，局部产生的血管紧张素Ⅱ释放的[β] 2-肾上腺素受体激活。β-肾上腺素受体激动剂和血管肾素-血管紧张素系统可能对外周血管张力的调节起重要作用。
The effect of a [beta]-adrenoceptor agonist on the release of the components of the vascular renin-angiotensin system was examined in vitro. Isolated rat mesenteric arteries were perfused in an open system with Krebs-Ringer solution and released immunoreactive angiotensin II (ANG IIir) into the perfusate was directly determined using a Sep-Pak C-18 cartridge connected to the perfusion system. Renin activity in the concentrated perfusate was also determined. Isoproterenol (1 nM-1 [mu] M) increased the release of ANG IIir in a dose-dependent manner. The increase in ANG IIir release during isoproterenol (1 [mu] M) infusion was inhibited by propranolol (1 [mu] M) or captopril (2 [mu] M). Isopro-terenol-induced increment of ANG IIir release was blocked by the selective [beta] 2-adrenoceptor antagonist, ICI 118,551 (1 [mu] M), but not by the selective [beta] 1-adrenoceptor antagonist, atenolol (1 [mu] M). Renin activity in the perfusate was measurable, but did not increase in response to isoproterenol (1 [mu] M) infusion. There was no significant difference in the response of ANG IIir release to isoproterenol between spontaneously hypertensive rats and Wistar-Kyoto rats. The present results indicate that locally generated ANG II is released by [beta] 2-adrenoceptor activation. The [beta]-adrenoceptor agonist and the vascular renin-angiotensin system may play an important role for the regulation of peripheral vascular tone.