In vitro effect of histamine and histamine H-1 and H-2 receptor antagonists on cellular proliferation of human malignant melanoma cell lines
In vitro effect of histamine and histamine H-1 and H-2 receptor antagonists on cellular proliferation of human malignant melanoma cell lines
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DOI：
10.1097/00008390-199604000-00003
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发表时间：
1996-04-01
影响因子：
2.2
通讯作者：
Morris, DL
中科院分区：
文献类型：
--
作者：
Reynolds, JL;Akhter, J;Morris, DL
组胺是胃癌和结直肠癌的公认生长因子。关于组胺对黑色素瘤的作用，已有报道，但有矛盾的数据。我们的目的是确定组胺和H-1和H-2受体拮抗剂对细胞生长和环AMP产生的影响。将四种人黑素瘤细胞系与一系列浓度的组胺，以及与H-2受体拮抗剂西咪替丁、雷尼替丁或法莫替丁，或H-1受体拮抗剂苯海拉明一起培养。通过[H-3]-胸苷的摄取来测量细胞增殖，还测量环AMP产生以确定细胞系的受体状态，组胺显著刺激四种细胞系中的两种的生长，最大刺激为1 × 10 ~（-8）M。所有四种拮抗剂均以剂量依赖性方式抑制该效应。组胺[10（-7）至10（-4）M]也诱导两种组胺反应细胞系中环AMP产生的剂量依赖性增加，表明这些细胞系表达H-2受体。我们的结论是，可能有一个作用，组胺受体拮抗剂在黑色素瘤的治疗和进一步的调查是必要的。
Histamine is an established growth factor for gastric and colorectal cancer. Contradictory data for response of melanoma to histamine have been reported, Our aims were to determine the effect of histamine and H-1 and H-2 receptor antagonists on cell growth and cyclic AMP production. Four human melanoma cell lines were cultured with a range of concentrations of histamine, and with the H-2 receptor antagonists cimetidine, ranitidine or famotidine, or the H-1 receptor antagonist diphenhydramine, Cellular proliferation was measured by the uptake of [H-3]-thymidine, Cyclic AMP production was also measured to determine the receptor status of the cell lines, Histamine significantly stimulated growth in two of four cell lines, with maximal stimulation at 1 x 10(-8) M, This effect was inhibited by all four antagonists in a dose-dependent manner. Histamine [10(-7) to 10(-4) M] also induced a dose dependent increase in cyclic AMP production in the two histamine-responsive cell lines, suggesting that these cell lines express H-2 receptors. We conclude that there may be a role for histamine receptor antagonists in melanoma treatment and that further investigation is warranted.