TREATMENT OF ABRIN AND RICIN WITH BETA-MERCAPTOETHANOL - OPPOSITE EFFECTS ON THEIR TOXICITY IN MICE AND THEIR ABILITY TO INHIBIT PROTEIN-SYNTHESIS IN A CELL-FREE SYSTEM
TREATMENT OF ABRIN AND RICIN WITH BETA-MERCAPTOETHANOL - OPPOSITE EFFECTS ON THEIR TOXICITY IN MICE AND THEIR ABILITY TO INHIBIT PROTEIN-SYNTHESIS IN A CELL-FREE SYSTEM
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DOI：
10.1016/0014-5793(72)80674-4
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发表时间：
1972-01-01
期刊：
影响因子：
3.5
通讯作者：
PIHL, A
中科院分区：
文献类型：
--
作者：
OLSNES, S;PIHL, A
最近，我们已经提出了证据表明，高毒性植物蛋白相思豆毒素和蓖麻毒素抑制从兔网织红细胞的无细胞系统中的肽链延长[1，21。我们的研究结果与其他作者的研究结果[3-61]一起表明，这两种蛋白质通过抑制蛋白质合成而在体内发挥其毒性作用。在本文中，我们希望报告，当用巯基乙醇处理相思豆毒素和蓖麻毒素时，它们在无细胞系统中抑制蛋白质合成的能力大大增加，同时它们在体内的毒性作用大大降低。蓖麻毒素和蓖麻毒素都由两条多肽链组成，通过二硫键连接在一起[2]，结果表明，观察到的效应是由于两条多肽链的分离，这两条多肽链可能具有不同的生物学功能。
Recently we have presented evidence that the highly toxic plant proteins abrin and ricin inhibit peptide chain elongation in a cell-free system from rabbit reticulocytes[1, 21. Our results, together with those of other authors [3-61, indicate that the two proteins exert their toxic effects in viva by inhibiting protein synthesis.In the present paper we wish to report that when abrin and ricin are treated with fl-mercaptoethanol, their ability to inhibit protein synthesis in a cell-free system is strongly increased, while concomitantly their toxic effects in viva are substantially reduced. Abrin and ricin both consist of two polypeptide chains held together by disulphide bonds [2], and the results suggest that the observed effects are due to separation of the two polypeptide chains which may have different biological functions.